Abstract
The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.
Original language | English (US) |
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Pages (from-to) | 121-123 |
Number of pages | 3 |
Journal | Zebrafish |
Volume | 5 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1 2008 |
ASJC Scopus subject areas
- Animal Science and Zoology
- Developmental Biology