TY - JOUR
T1 - Transfected synphilin-1 forms cytoplasmic inclusions in HEK293 cells
AU - O'Farrell, Casey
AU - Murphy, Diane D.
AU - Petrucelli, Leonard
AU - Singleton, Andrew B.
AU - Hussey, Jennifer
AU - Farrer, Matt
AU - Hardy, John
AU - Dickson, Dennis W.
AU - Cookson, Mark R.
N1 - Funding Information:
The authors would like to acknowledge the generous gifts of reagents for HSV-1 packaging from Dr. Rachael Neve, Harvard Medical School, Boston. This work was supported by NINDS grants P01-NS40256 and RO1-NS41816-01.
PY - 2001/12/16
Y1 - 2001/12/16
N2 - The discovery of mutations in the gene for α-synuclein in familial Parkinson's disease (PD) has led to an increased interest in this pre-synaptic protein. Synphilin-1, a potential synuclein-binding protein, was cloned using yeast two-hybrid assays. The function of synphilin-1 is currently unknown, although it has been reported to be present along with α-synuclein in Lewy bodies in PD. In the present study, we monitored synphilin-1 aggregation directly using fusion proteins of synphilin-1 and green fluorescent protein (EGFP). Transfection of synphilin-EGFP fusion proteins formed cytoplasmic inclusions in HEK293 cells. Although these inclusions overlapped with the distribution of α-synuclein, they were unlike Lewy bodies in that they were not eosinophilic, and instead were membrane-bound, lipid-rich cytoplasmic inclusions.
AB - The discovery of mutations in the gene for α-synuclein in familial Parkinson's disease (PD) has led to an increased interest in this pre-synaptic protein. Synphilin-1, a potential synuclein-binding protein, was cloned using yeast two-hybrid assays. The function of synphilin-1 is currently unknown, although it has been reported to be present along with α-synuclein in Lewy bodies in PD. In the present study, we monitored synphilin-1 aggregation directly using fusion proteins of synphilin-1 and green fluorescent protein (EGFP). Transfection of synphilin-EGFP fusion proteins formed cytoplasmic inclusions in HEK293 cells. Although these inclusions overlapped with the distribution of α-synuclein, they were unlike Lewy bodies in that they were not eosinophilic, and instead were membrane-bound, lipid-rich cytoplasmic inclusions.
KW - Green fluorescent protein
KW - Parkinson's disease
KW - Protein-protein interaction
KW - Synuclein
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U2 - 10.1016/S0169-328X(01)00292-3
DO - 10.1016/S0169-328X(01)00292-3
M3 - Article
C2 - 11744167
AN - SCOPUS:0035900882
SN - 0169-328X
VL - 97
SP - 94
EP - 102
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -