Molecular Characterization of a Putative Antiretroviral Transcriptional Factor, OTK18

Kimberly A. Carlson, Gary Leisman, Jenae Limoges, Garrett D. Pohlman, Masahide Horiba, James Buescher, Howard E. Gendelman, Tsuneya Ikezu

Research output: Contribution to journalArticlepeer-review


Elucidation of the factors involved in host defense against human immunodeficiency viral infection remains pivotal if viral control may be achieved. Toward these ends, we investigated the function of a putative antiretroviral factor, OTK18, isolated by differential display of mRNA from HIV type 1-infected primary human monocyte-derived macrophages. Molecular and immunohistochemical analyses showed that the OTK18 nucleotide sequence contains 13 adjacent C2H2-type zinc finger motifs, a Krüppel-associated box, and is localized to both cytosol and nucleus. Mutational analyses revealed that both the Krüppel-associated box and zinc finger regions of OTK18 are responsible for the transcriptional suppressive activities of this gene. OTK18 was copiously expressed in macrophages following HIV type 1 infection and diminished progeny virion production. A mechanism for this antiretroviral activity was by suppression of HIV type 1 Tat-induced viral long terminal repeat promoter activity. Our findings suggest that one possible function of OTK18 is as a HIV type 1-inducible transcriptional suppresser.

Original languageEnglish (US)
Pages (from-to)381-391
Number of pages11
JournalJournal of Immunology
Issue number1
StatePublished - Jan 1 2004

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Molecular Characterization of a Putative Antiretroviral Transcriptional Factor, OTK18'. Together they form a unique fingerprint.

Cite this