A chemical strategy to study transient states in substrate ubiquitylation by cryo-EM

Qi Hu; Maria Victoria Botuyan; Georges Mer

doi:10.1016/j.chempr.2023.04.016

A chemical strategy to study transient states in substrate ubiquitylation by cryo-EM

Qi Hu, Maria Victoria Botuyan, Georges Mer

Biochemistry and Molecular Biology

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

Characterization of short-lived intermediate states along the path of a ubiquitylation reaction remains challenging. In this issue of Chem, Ai et al. present a chemical trapping method for probing transient intermediates in substrate ubiquitylation. The usefulness of this approach is demonstrated by the determination of single-particle cryoelectron microscopy (cryo-EM) structures related to nucleosome ubiquitylation.

Original language	English (US)
Pages (from-to)	1069-1071
Number of pages	3
Journal	Chem
Volume	9
Issue number	5
DOIs	https://doi.org/10.1016/j.chempr.2023.04.016
State	Published - May 11 2023

ASJC Scopus subject areas

General Chemistry
Biochemistry
Environmental Chemistry
General Chemical Engineering
Biochemistry, medical
Materials Chemistry

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10.1016/j.chempr.2023.04.016

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title = "A chemical strategy to study transient states in substrate ubiquitylation by cryo-EM",

abstract = "Characterization of short-lived intermediate states along the path of a ubiquitylation reaction remains challenging. In this issue of Chem, Ai et al. present a chemical trapping method for probing transient intermediates in substrate ubiquitylation. The usefulness of this approach is demonstrated by the determination of single-particle cryoelectron microscopy (cryo-EM) structures related to nucleosome ubiquitylation.",

author = "Qi Hu and Botuyan, {Maria Victoria} and Georges Mer",

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AU - Mer, Georges

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N2 - Characterization of short-lived intermediate states along the path of a ubiquitylation reaction remains challenging. In this issue of Chem, Ai et al. present a chemical trapping method for probing transient intermediates in substrate ubiquitylation. The usefulness of this approach is demonstrated by the determination of single-particle cryoelectron microscopy (cryo-EM) structures related to nucleosome ubiquitylation.

AB - Characterization of short-lived intermediate states along the path of a ubiquitylation reaction remains challenging. In this issue of Chem, Ai et al. present a chemical trapping method for probing transient intermediates in substrate ubiquitylation. The usefulness of this approach is demonstrated by the determination of single-particle cryoelectron microscopy (cryo-EM) structures related to nucleosome ubiquitylation.

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A chemical strategy to study transient states in substrate ubiquitylation by cryo-EM

Abstract

ASJC Scopus subject areas

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