Whole cell electrophysiology of primary cultured murine enterochromaffin cells

Katilyn Knutson, Peter R. Strege, Joyce Li, Andrew B. Leiter, Gianrico Farrugia, Arthur Beyder

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Enterochromaffin (EC) cells in the gastrointestinal (GI) epithelium constitute the largest subpopulation of enteroendocrine cells. As specialized sensory cells, EC cells sense luminal stimuli and convert them into serotonin (5-hyroxytryptamine, 5-HT) release events. However, the electrophysiology of these cells is poorly understood because they are difficult to culture and to identify. The method presented in this paper outlines primary EC cell cultures optimized for single cell electrophysiology. This protocol utilizes a transgenic cyan fluorescent protein (CFP) reporter to identify mouse EC cells in mixed primary cultures, advancing the approach to obtaining high-quality recordings of whole cell electrophysiology in voltage-and current-clamp modes.

Original languageEnglish (US)
Article numbere58112
JournalJournal of Visualized Experiments
Issue number139
StatePublished - Sep 26 2018


  • Biology
  • Current clamp
  • Enterochromaffin cell
  • Epithelium
  • Gastrointestinal
  • Issue 139
  • Primary culture
  • Voltage clamp
  • Whole cell electrophysiology

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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