VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis

Taro Matsumoto, Svante Bohman, Johan Dixelius, Tone Berge, Anna Dimberg, Peetra Magnusson, Ling Wang, Charlotte Wikner, Jian Hua Qi, Christer Wernstedt, Jiong Wu, Skjalg Bruheim, Hideo Mugishima, Debrabata Mukhopadhyay, Anne Spurkland, Lena Claesson-Welsh

Research output: Contribution to journalArticlepeer-review

201 Scopus citations


Vascular endothelial growth factor receptor-2 (VEGFR-2) activation by VEGF-A is essential in vasculogenesis and angiogenesis. We have generated a pan-phosphorylation site map of VEGFR-2 and identified one major tyrosine phosphorylation site in the kinase insert (Y951), in addition to two major sites in the C-terminal tail (Y1175 and Y1214). In developing vessels, phosphorylation of Y1175 and Y1214 was detected in all VEGFR-2-expressing endothelial cells, whereas phosphorylation of Y951 was identified in a subset of vessels. Phosphorylated Y951 bound the T-cell-specific adapter (TSAd), which was expressed in tumor vessels. Mutation of Y951 to F and introduction of phosphorylated Y951 peptide or TSAd siRNA into endothelial cells blocked VEGF-A-induced actin stress fibers and migration, but not mitogenesis. Tumor vascularization and growth was reduced in TSAd-deficient mice, indicating a critical role of Y951-TSAd signaling in pathological angiogenesis.

Original languageEnglish (US)
Pages (from-to)2342-2353
Number of pages12
JournalEMBO Journal
Issue number13
StatePublished - Jul 6 2005


  • Actin cytoskeleton
  • TSAd
  • Tumor angiogenesis
  • Tyrosine phosphorylation site
  • VEGFR-2

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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