Abstract
Multi-color flow cytometry is a unique technology, which enables the analysis of heterogeneous cellular systems and provides multiparametric information on a cell-by-cell basis. A variety of factors contribute to the complexity of validating cell-based flow cytometric methods, including the lack of fully characterized cellular reference materials and the difficulty in obtaining, or creating, samples with varying levels of a given cell type or varying levels of expression of a given antigen. This document summarizes validation requirements and describes validation strategies for quasi-quantitative and qualitative cell-based flow cytometric assays.
Original language | English (US) |
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Pages (from-to) | 315-323 |
Number of pages | 9 |
Journal | Cytometry Part B - Clinical Cytometry |
Volume | 84 |
Issue number | 5 |
DOIs | |
State | Published - Sep 2013 |
Keywords
- Cytometry
- accuracy
- imprecision
- sensitivity
- specificity
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology
- Cell Biology