Universal primers can amplify tumor necrosis factor gene across species

Universal primers designed in the conserved regions of sequences can be used for cross-species gene amplification and for identification of unsequenced genes from other species. We have designed a universal primer for the tumor necrosis factor gene. This has been tested for the amplification of tumor necrosis factor gene from monkey and guinea pig for which DNA sequence is not yet known. The universal primer thus designed can be used for identification and expression studies for tumor necrosis factor genes in other unknown species. The strategy showed in this paper can be used in designing universal primers for the study of various subjects involving unsequenced DNA.