Trefoil factor-1 (TFF1) enhances oncogenicity of mammary carcinoma cells

Naeem Amiry, Xiangjun Kong, Nethaji Muniraj, Nagarajan Kannan, Prudence M. Grandison, Juan Lin, Yulu Yang, Cécile M. Vouyovitch, Sahra Borges, Jo K. Perry, Hichem C. Mertani, Tao Zhu, Dongxu Liu, Peter E. Lobie

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


The functional role of autocrine trefoil factor-1 (TFF1) in mammary carcinoma has not been previously elucidated. Herein, we demonstrate that forced expression of TFF1 in mammary carcinoma cells resulted in increased total cell number as a consequence of increased cell proliferation and survival. Forced expression of TFF1 enhanced anchorage-independent growth and promoted scattered cell morphology with increased cell migration and invasion. Moreover, forced expression of TFF1 increased tumor size in xenograft models. Conversely, RNA interference-mediated depletion of TFF1 in mammary carcinoma cells significantly reduced anchorage-independent growth and migration. Furthermore, neutralization of secreted TFF1 protein by polyclonal antibody decreased mammary carcinoma cell viability in vitro and resulted in regression of mammary carcinoma xenografts. We have therefore demonstrated that TFF1 possesses oncogenic functions in mammary carcinoma cells. Functional antagonism of TFF1 can therefore be considered as a novel therapeutic strategy for mammary carcinoma.

Original languageEnglish (US)
Pages (from-to)4473-4483
Number of pages11
Issue number10
StatePublished - Oct 2009

ASJC Scopus subject areas

  • Endocrinology


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