Transgenesis in Mouse Embryonic Stem Cells

Janine van Ree, Wei Zhou, Ming Li, Jan M. van Deursen

Research output: Chapter in Book/Report/Conference proceedingChapter


Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, ­promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages20
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cre recombinase
  • Doxycycline
  • ES cells
  • Inducible expression
  • Transgenic mice

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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