Transforming growth factor-b (TGF-β) expression is increased in the subsynovial connective tissue in a rabbit model of carpal tunnel syndrome

Takako Chikenji; Anne Gingery; Chunfeng Zhao; Matthias Vanhees; Tamami Moriya; Ramona Reisdorf; Kai Nan An; Peter C. Amadio

doi:10.1371/journal.pone.0108312

Transforming growth factor-b (TGF-β) expression is increased in the subsynovial connective tissue in a rabbit model of carpal tunnel syndrome

Takako Chikenji, Anne Gingery, Chunfeng Zhao, Matthias Vanhees, Tamami Moriya, Ramona Reisdorf, Kai Nan An, Peter C. Amadio

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Carpal tunnel syndrome (CTS) is an idiopathic disease that results from increased fibrosis of the subsynovial connective tissue (SSCT). A recent study found overexpression of both transforming growth factor-b (TGF-β) and connective tissue growth factor (CTGF) in the SSCT of CTS patients. This study investigated TGF-β and CTGF expression in a rabbit model of CTS, in which SSCT fibrosis is induced by a surgical injury. Levels of TGF-β1 and CTGF at 6, 12, 24 weeks after injury were determined by immunohistochemistry A significant increase in TGF-β1 and a concomitant significant increase in CTGF were found at 6 weeks, in addition to higher cell density compared to normal (all p<0.05), Interestingly, CTGF expression was reduced at 12 and 24 weeks, suggesting that an initial insult results in a time limited response. We conclude that this rabbit model mimics the fibrosis found in human CTS, and may be useful to study pathogenetic mechanisms of CTS in vivo.

Original language	English (US)
Article number	e108312
Journal	PloS one
Volume	9
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0108312
State	Published - Sep 30 2014

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

Access to Document

10.1371/journal.pone.0108312

Cite this

@article{e8ad8bb92d7b40c197baff7f6d6f3ff8,

title = "Transforming growth factor-b (TGF-β) expression is increased in the subsynovial connective tissue in a rabbit model of carpal tunnel syndrome",

abstract = "Carpal tunnel syndrome (CTS) is an idiopathic disease that results from increased fibrosis of the subsynovial connective tissue (SSCT). A recent study found overexpression of both transforming growth factor-b (TGF-β) and connective tissue growth factor (CTGF) in the SSCT of CTS patients. This study investigated TGF-β and CTGF expression in a rabbit model of CTS, in which SSCT fibrosis is induced by a surgical injury. Levels of TGF-β1 and CTGF at 6, 12, 24 weeks after injury were determined by immunohistochemistry A significant increase in TGF-β1 and a concomitant significant increase in CTGF were found at 6 weeks, in addition to higher cell density compared to normal (all p<0.05), Interestingly, CTGF expression was reduced at 12 and 24 weeks, suggesting that an initial insult results in a time limited response. We conclude that this rabbit model mimics the fibrosis found in human CTS, and may be useful to study pathogenetic mechanisms of CTS in vivo.",

author = "Takako Chikenji and Anne Gingery and Chunfeng Zhao and Matthias Vanhees and Tamami Moriya and Ramona Reisdorf and An, {Kai Nan} and Amadio, {Peter C.}",

note = "Publisher Copyright: {\textcopyright} 2014 Chikenji et al.",

year = "2014",

month = sep,

day = "30",

doi = "10.1371/journal.pone.0108312",

language = "English (US)",

volume = "9",

journal = "PloS one",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "9",

}

TY - JOUR

T1 - Transforming growth factor-b (TGF-β) expression is increased in the subsynovial connective tissue in a rabbit model of carpal tunnel syndrome

AU - Chikenji, Takako

AU - Gingery, Anne

AU - Zhao, Chunfeng

AU - Vanhees, Matthias

AU - Moriya, Tamami

AU - Reisdorf, Ramona

AU - An, Kai Nan

AU - Amadio, Peter C.

PY - 2014/9/30

Y1 - 2014/9/30

N2 - Carpal tunnel syndrome (CTS) is an idiopathic disease that results from increased fibrosis of the subsynovial connective tissue (SSCT). A recent study found overexpression of both transforming growth factor-b (TGF-β) and connective tissue growth factor (CTGF) in the SSCT of CTS patients. This study investigated TGF-β and CTGF expression in a rabbit model of CTS, in which SSCT fibrosis is induced by a surgical injury. Levels of TGF-β1 and CTGF at 6, 12, 24 weeks after injury were determined by immunohistochemistry A significant increase in TGF-β1 and a concomitant significant increase in CTGF were found at 6 weeks, in addition to higher cell density compared to normal (all p<0.05), Interestingly, CTGF expression was reduced at 12 and 24 weeks, suggesting that an initial insult results in a time limited response. We conclude that this rabbit model mimics the fibrosis found in human CTS, and may be useful to study pathogenetic mechanisms of CTS in vivo.

AB - Carpal tunnel syndrome (CTS) is an idiopathic disease that results from increased fibrosis of the subsynovial connective tissue (SSCT). A recent study found overexpression of both transforming growth factor-b (TGF-β) and connective tissue growth factor (CTGF) in the SSCT of CTS patients. This study investigated TGF-β and CTGF expression in a rabbit model of CTS, in which SSCT fibrosis is induced by a surgical injury. Levels of TGF-β1 and CTGF at 6, 12, 24 weeks after injury were determined by immunohistochemistry A significant increase in TGF-β1 and a concomitant significant increase in CTGF were found at 6 weeks, in addition to higher cell density compared to normal (all p<0.05), Interestingly, CTGF expression was reduced at 12 and 24 weeks, suggesting that an initial insult results in a time limited response. We conclude that this rabbit model mimics the fibrosis found in human CTS, and may be useful to study pathogenetic mechanisms of CTS in vivo.

UR - http://www.scopus.com/inward/record.url?scp=84907481629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907481629&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0108312

DO - 10.1371/journal.pone.0108312

M3 - Article

C2 - 25269071

AN - SCOPUS:84907481629

SN - 1932-6203

VL - 9

JO - PloS one

JF - PloS one

IS - 9

M1 - e108312

ER -

Transforming growth factor-b (TGF-β) expression is increased in the subsynovial connective tissue in a rabbit model of carpal tunnel syndrome

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this