Abstract
The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.
Original language | English (US) |
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Pages (from-to) | 387-390 |
Number of pages | 4 |
Journal | Molecular and cellular biology |
Volume | 10 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1990 |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology