TY - JOUR
T1 - Thyroxine-binding antibodies inhibit T cell recognition of a pathogenic thyroglobulin epitope
AU - Dai, Yang D.
AU - Eliades, Petros
AU - Carayanniotis, Karen A.
AU - McCormick, Daniel J.
AU - Kong, Yi Chi M.
AU - Magafa, Vassiliki
AU - Cordopatis, Paul
AU - Lymberi, Peggy
AU - Carayanniotis, George
PY - 2005/3/1
Y1 - 2005/3/1
N2 - Thyroid hormone-binding (THB) Abs are frequently detected in autoimmune thyroid disorders but it is unknown whether they can exert immunoregulatory effects. We report that a THB mAb recognizing the 5′ iodine atom of the outer phenolic ring of thyroxine (T4) can block T cell recognition of the pathogenic thyroglobulin (Tg) peptide (2549-2560) that contains T4 at aa position 2553 (T4(2553)). Following peptide binding to the MHC groove, the THB mAb inhibited activation of the Ak-restricted, T4(2553)-specific, mouse T cell hybridoma clone 3.47, which does not recognize other T4-containing epitopes or noniodinated peptide analogues. Addition of the same THB mAb to T4(2553)-pulsed splenocytes largely inhibited specific activation of T4(2553)-primed lymph node cells and significantly reduced their capacity to adoptively transfer thyroiditis to naive CBA/J mice. These data demonstrate that some THB Abs can block recognition of iodine-containing Tg epitopes by autoaggressive T cells and support the view that such Abs may influence the development or maintenance of thyroid disease.
AB - Thyroid hormone-binding (THB) Abs are frequently detected in autoimmune thyroid disorders but it is unknown whether they can exert immunoregulatory effects. We report that a THB mAb recognizing the 5′ iodine atom of the outer phenolic ring of thyroxine (T4) can block T cell recognition of the pathogenic thyroglobulin (Tg) peptide (2549-2560) that contains T4 at aa position 2553 (T4(2553)). Following peptide binding to the MHC groove, the THB mAb inhibited activation of the Ak-restricted, T4(2553)-specific, mouse T cell hybridoma clone 3.47, which does not recognize other T4-containing epitopes or noniodinated peptide analogues. Addition of the same THB mAb to T4(2553)-pulsed splenocytes largely inhibited specific activation of T4(2553)-primed lymph node cells and significantly reduced their capacity to adoptively transfer thyroiditis to naive CBA/J mice. These data demonstrate that some THB Abs can block recognition of iodine-containing Tg epitopes by autoaggressive T cells and support the view that such Abs may influence the development or maintenance of thyroid disease.
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U2 - 10.4049/jimmunol.174.5.3105
DO - 10.4049/jimmunol.174.5.3105
M3 - Article
C2 - 15728526
AN - SCOPUS:14044273646
SN - 0022-1767
VL - 174
SP - 3105
EP - 3110
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -