The transition of zebrafish functional genetics from random mutagenesis to targeted integration

Louis Y.El Khoury; Jarryd M. Campbell; Karl J. Clark

doi:10.1016/B978-0-12-804078-2.00017-9

The transition of zebrafish functional genetics from random mutagenesis to targeted integration

Louis Y.El Khoury, Jarryd M. Campbell, Karl J. Clark

Biochemistry and Molecular Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

The zebrafish is a popular animal model for the study of various aspects of health and disease due to its high fecundity, rapid embryonic development, relatively low maintenance costs, and increasing genetic malleability. In light of the high genetic tractability of zebrafish and the constant developments observed in the field of genetic engineering, this chapter reviews some of the major random and targeted gene-editing methods employed in the generation of a variety of mutant and transgenic fish lines to better understand genotype-phenotype relationships. Furthermore, this chapter proposes a novel zebrafish larvae mutagenesis and genotyping approach by coupling targeted integrations with mutagenic gene-break transposon vectors containing fluorescent reporters.

Original language	English (US)
Title of host publication	Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research
Publisher	Elsevier
Pages	401-416
Number of pages	16
ISBN (Electronic)	9780128040782
ISBN (Print)	9780128041161
DOIs	https://doi.org/10.1016/B978-0-12-804078-2.00017-9
State	Published - Jan 1 2018

Keywords

CRISPR
Gene editing
Gene traps
Gene-break transposons
Genetic engineering
Homology-directed repair (HDR)
Nonhomologous end joining (NHEJ)
TALEN
ZFN
Zebrafish

ASJC Scopus subject areas

General Medicine
General Neuroscience

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10.1016/B978-0-12-804078-2.00017-9

Cite this

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AU - Clark, Karl J.

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AB - The zebrafish is a popular animal model for the study of various aspects of health and disease due to its high fecundity, rapid embryonic development, relatively low maintenance costs, and increasing genetic malleability. In light of the high genetic tractability of zebrafish and the constant developments observed in the field of genetic engineering, this chapter reviews some of the major random and targeted gene-editing methods employed in the generation of a variety of mutant and transgenic fish lines to better understand genotype-phenotype relationships. Furthermore, this chapter proposes a novel zebrafish larvae mutagenesis and genotyping approach by coupling targeted integrations with mutagenic gene-break transposon vectors containing fluorescent reporters.

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KW - Gene editing

KW - Gene traps

KW - Gene-break transposons

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The transition of zebrafish functional genetics from random mutagenesis to targeted integration

Abstract

Keywords

ASJC Scopus subject areas

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