TY - JOUR
T1 - The prostate, lung, colorectal and ovarian cancer (PLCO) screening trial pathology tissue resource
AU - Zhu, Claire S.
AU - Huang, Wen Yi
AU - Pinsky, Paul F.
AU - Berg, Christine D.
AU - Sherman, Mark
AU - Yu, Kelly J.
AU - Carrick, Danielle M.
AU - Black, Amanda
AU - Hoover, Robert
AU - Lenz, Petra
AU - Williams, Craig
AU - Hawkins, Laura
AU - Chaloux, Matthew
AU - Yurgalevitch, Susan
AU - Mathew, Sunitha
AU - Miller, Amy
AU - Olivo, Vanessa
AU - Khan, Asia
AU - Pretzel, Shannon M.
AU - Multerer, Deborah
AU - Beckmann, Patricia
AU - Broski, Karen G.
AU - Freedman, Neal D.
N1 - Publisher Copyright:
© 2016 AACR.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Background: Pathology tissue specimens with associated epidemiologic and clinical data are valuable for cancer research. The Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial undertook a large-scale effort to create a public resource of pathology tissues from PLCO participants who developed a cancer during the trial. Methods: Formalin-fixed paraffin-embedded tissue blocks were obtained from pathology laboratories on a loan basis for central processing of tissue microarrays, with additional free-standing tissue cores collected for nucleic acid extraction. Results: Pathology tissue specimens were obtained for prostate cancer (n = 1,052), lung cancer (n = 434), colorectal cancer (n=675) and adenoma (n=658), ovarian cancer and borderline tumors (n = 212), breast cancer (n = 870), and bladder cancer (n = 204). The process of creating this resource was complex, involving multidisciplinary teams with expertise in pathology, epidemiology, information technology, project management, and specialized laboratories. Conclusions: Creating the PLCO tissue resource required a multistep process, including obtaining medical records and contacting pathology departments where pathology materials were stored after obtaining necessary patient consent and authorization. The potential to link tissue biomarkers to prospectively collected epidemiologic information, screening and clinical data, and matched blood or buccal samples offers valuable opportunities to study etiologic heterogeneity, mechanisms of carcinogenesis, and biomarkers for early detection and prognosis. Impact: The methods and protocols developed for this effort, and the detailed description of this resource provided here, will be useful for those seeking to use PLCO pathology tissue specimens for their research and may also inform future tissue collection efforts in other settings.
AB - Background: Pathology tissue specimens with associated epidemiologic and clinical data are valuable for cancer research. The Prostate, Lung, Colorectal and Ovarian (PLCO) Cancer Screening Trial undertook a large-scale effort to create a public resource of pathology tissues from PLCO participants who developed a cancer during the trial. Methods: Formalin-fixed paraffin-embedded tissue blocks were obtained from pathology laboratories on a loan basis for central processing of tissue microarrays, with additional free-standing tissue cores collected for nucleic acid extraction. Results: Pathology tissue specimens were obtained for prostate cancer (n = 1,052), lung cancer (n = 434), colorectal cancer (n=675) and adenoma (n=658), ovarian cancer and borderline tumors (n = 212), breast cancer (n = 870), and bladder cancer (n = 204). The process of creating this resource was complex, involving multidisciplinary teams with expertise in pathology, epidemiology, information technology, project management, and specialized laboratories. Conclusions: Creating the PLCO tissue resource required a multistep process, including obtaining medical records and contacting pathology departments where pathology materials were stored after obtaining necessary patient consent and authorization. The potential to link tissue biomarkers to prospectively collected epidemiologic information, screening and clinical data, and matched blood or buccal samples offers valuable opportunities to study etiologic heterogeneity, mechanisms of carcinogenesis, and biomarkers for early detection and prognosis. Impact: The methods and protocols developed for this effort, and the detailed description of this resource provided here, will be useful for those seeking to use PLCO pathology tissue specimens for their research and may also inform future tissue collection efforts in other settings.
UR - http://www.scopus.com/inward/record.url?scp=85006287974&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85006287974&partnerID=8YFLogxK
U2 - 10.1158/1055-9965.EPI-16-0506
DO - 10.1158/1055-9965.EPI-16-0506
M3 - Article
C2 - 27635065
AN - SCOPUS:85006287974
SN - 1055-9965
VL - 25
SP - 1635
EP - 1642
JO - Cancer Epidemiology Biomarkers and Prevention
JF - Cancer Epidemiology Biomarkers and Prevention
IS - 12
ER -