The Dynamic and Crucial Role of the Arginine Methylproteome in Myoblast Cell Differentiation

Nikolaos A. Papanikolaou, Marios Nikolaidis, Grigorios D. Amoutzias, Ariadni Fouza, Maria Papaioannou, Akhilesh Pandey, Athanasios G. Papavassiliou

Research output: Contribution to journalArticlepeer-review


Protein arginine methylation is an extensive and functionally significant post-translational modification. However, little is known about its role in differentiation at the systems level. Using stable isotope labeling by amino acids in cell culture (SILAC) proteomics of whole proteome analysis in proliferating or five-day differentiated mouse C2C12 myoblasts, followed by high-resolution mass spectrometry, biochemical assays, and specific immunoprecipitation of mono- or dimethylated arginine peptides, we identified several protein families that were differentially methylated on arginine. Our study is the first to reveal global changes in the arginine mono- or dimethylation of proteins in proliferating myoblasts and differentiated myocytes and to identify enriched protein domains and novel short linear motifs (SLiMs). Our data may be crucial for dissecting the links between differentiation and cancer growth.

Original languageEnglish (US)
Article number2124
JournalInternational journal of molecular sciences
Issue number3
StatePublished - Feb 2023


  • C2C12
  • Short Linear Motifs (SliMs)
  • arginine
  • differentiation
  • methylproteome
  • myoblast

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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