The COUP-adjacent repressor (CAR) element participates in the tissue-specific expression of the ovalbumin gene

David G. Monroe, Michel M. Sanders

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The ovalbumin (Ov) gene is an excellent model for the study of tissue-specific gene regulation as it is only active in the estrogen-stimulated oviduct. Previous studies have demonstrated that the negative regulatory element (NRE) in the Ov gene 5'-flanking region is responsible for silencing the gene in oviduct in the absence of steroids. Linker scanning analysis defined an element within the NRE designated the COUP-adjacent repressor (CAR) element as a repressor of Ov gene expression. However, the role of the CAR element in non-oviduct tissues has not been addressed. Using transient transfection analysis of various Ov 5'-flanking region constructs into the estrogen-responsive chicken hepatocyte cell line LMH/2A, we demonstrate that Ov gene expression is not induced by estrogen and that an active repressor element exists in the NRE. Deletion analysis indicates that the region from -134 to -87, which includes the CAR element, mediates this repression. Mutation of the CAR element relieves repression, leading to high levels of gene expression. These data support a model where the inhibition of Ov gene expression in non-oviduct cells is a combination of the lack of essential positive factors and the presence of an active repressor, which binds to the CAR element.

Original languageEnglish (US)
Pages (from-to)27-32
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Issue number1
StatePublished - Dec 15 2000


  • Estrogen
  • Ovalbumin
  • Repression
  • Tissue-specific regulation

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics


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