TY - JOUR
T1 - Targeting self- and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells
AU - Li, Dapeng
AU - Romain, Gabrielle
AU - Flamar, Anne Laure
AU - Duluc, Dorothée
AU - Dullaers, Melissa
AU - Li, Xiao Hua
AU - Zurawski, Sandra
AU - Bosquet, Nathalie
AU - Palucka, Anna Karolina
AU - Le Grand, Roger
AU - O'Garra, Anne
AU - Zurawski, Gerard
AU - Banchereau, Jacques
AU - Oh, Sang Kon
PY - 2012/1
Y1 - 2012/1
N2 - Dendritic cells (DCs) can initiate and shape host immune responses toward either immunity or tolerance by their effects on antigen-specific CD4+ T cells. DC-asialoglycoprotein receptor (DC-ASGPR), a lectinlike receptor, is a known scavenger receptor. Here, we report that targeting antigens to human DCs via DC-ASGPR, but not lectin-like oxidized-LDL receptor, Dectin-1, or DC-specific ICAM-3-grabbing nonintegrin favors the generation of antigen-specific suppressive CD4+ T cells that produce interleukin 10 (IL-10). These findings apply to both self- and foreign antigens, as well as memory and naive CD4+ T cells. The generation of such IL-10-producing CD4+ T cells requires p38/extracellular signal-regulated kinase phosphorylation and IL-10 induction in DCs. We further demonstrate that immunization of nonhuman primates with antigens fused to anti-DC-ASGPR monoclonal antibody generates antigen-specific CD4+ T cells that produce IL-10 in vivo. This study provides a new strategy for the establishment of antigen-specific IL-10- producing suppressive T cells in vivo by targeting whole protein antigens to DCs via DC-ASGPR.
AB - Dendritic cells (DCs) can initiate and shape host immune responses toward either immunity or tolerance by their effects on antigen-specific CD4+ T cells. DC-asialoglycoprotein receptor (DC-ASGPR), a lectinlike receptor, is a known scavenger receptor. Here, we report that targeting antigens to human DCs via DC-ASGPR, but not lectin-like oxidized-LDL receptor, Dectin-1, or DC-specific ICAM-3-grabbing nonintegrin favors the generation of antigen-specific suppressive CD4+ T cells that produce interleukin 10 (IL-10). These findings apply to both self- and foreign antigens, as well as memory and naive CD4+ T cells. The generation of such IL-10-producing CD4+ T cells requires p38/extracellular signal-regulated kinase phosphorylation and IL-10 induction in DCs. We further demonstrate that immunization of nonhuman primates with antigens fused to anti-DC-ASGPR monoclonal antibody generates antigen-specific CD4+ T cells that produce IL-10 in vivo. This study provides a new strategy for the establishment of antigen-specific IL-10- producing suppressive T cells in vivo by targeting whole protein antigens to DCs via DC-ASGPR.
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U2 - 10.1084/jem.20110399
DO - 10.1084/jem.20110399
M3 - Article
C2 - 22213806
AN - SCOPUS:84863116620
SN - 0022-1007
VL - 209
SP - 109
EP - 121
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 1
ER -