Abstract
Intramolecular correlations among the 18O-labels of metabolic oligophosphates, mapped by J-decoupled 31P NMR 2D chemical shift correlation spectroscopy, impart stringent constraints to the 18O-isotope distributions over the whole oligophosphate moiety. The multiple deduced correlations of isotopic labels enable determination of site-specific fractional isotope enrichments and unravel the isotopologue statistics. This approach ensures accurate determination of 18O- labeling rates of phosphometabolites, critical in biochemical energy conversion and metabolic flux transmission. The biological usefulness of the J-decoupled 31P NMR 2D chemical shift correlation maps was validated on adenosine tri-phosphate fractionally 18O labeled in perfused mammalian hearts.
Original language | English (US) |
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Pages (from-to) | 237-245 |
Number of pages | 9 |
Journal | Journal of Biomolecular NMR |
Volume | 50 |
Issue number | 3 |
DOIs | |
State | Published - Jul 2011 |
Keywords
- ATP turnover
- High-energy phosphoryl dynamics
- Isotopologue distribution
- O/O isotopic effects
- Oxygen-18
- P NMR chemical shift correlation
- Phosphometabolomics
- Phosphotransfer
ASJC Scopus subject areas
- Biochemistry
- Spectroscopy