31P NMR assessment of orthotopic liver rejection in a rat model

John L. Bowers, Katsunori Kawano, Kenneth R. Metz, Kenichi Teramoto, Ann McCullough, Melvin E. Clouse

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1 Scopus citations


31P NMR spectroscopy was used serially to study rejecting (ACI→LEW) and nonrejecting (ACl→ACl) orthotopic liver transplants in rats. Recipients were evaluated on post‐transplant days 1,3, 5, 7, 9, and 11. The relative changes in phosphomonoester (PME), inorganic phosphate (Pi), high‐energy phosphates and pH were studied. The earliest significant difference between the rejecting and nonrejecting groups was a decrease in the α‐NTP peak area on Day 5. This was followed by significant decreases in β‐NTP and pH, and increases in PME and Pi on Day 7. High‐resolution 31P NMR spectra of perchloric acid extracts demonstrated the PME increase to be due mainly to elevated phosphoethanolamine. Using the parameter (Pi + PME)/(α + β + γ‐NTP), rejecting livers were distinguished from nonrejecting livers at a moderate stage of rejection.

Original languageEnglish (US)
Pages (from-to)164-169
Number of pages6
JournalMagnetic Resonance in Medicine
Issue number2
StatePublished - Aug 1994


  • P NMR
  • liver
  • rejection
  • transplantation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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