Abstract
Radiolabelled leukocytes are useful for the imaging of inflammation and infection, and 18F-fluorodeoxyglucose (18F-FDG) is known to concentrate in metabolically active cells. We evaluated the feasibility of leukocyte labelling with 18F-FDG using ACD and heparin anticoagulants at 20°C and 37°C, with and without gentle mixing during incubation. With leukocytes (WBC) harvested from 20 ml blood, studies were performed using 18F-FDG in concentrations of 3.7-74 MBq (0.1-2.0 mCi). 18F-FDG WBC stability in platelet-poor plasma was assessed at 1-4 h. Satisfactory labelling efficiency was achieved with incubation in heparin-saline at 37°C for 30 min (62.7±1.6%), and was further enhanced by mixing during incubation (78.1±3.9%). Cell labelling was predominantly of granulocytes (78.5±1.4%). 18F-FDG WBC was relatively stable in platelet-poor plasma for up to 4 h, and no cell staining was observed in viability studies using trypan blue. These results indicate the feasibility of leukocyte labelling with 18F-FDG, providing an approach that may be useful in PET imaging of inflammation and infection. (
Original language | English (US) |
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Pages (from-to) | 691-694 |
Number of pages | 4 |
Journal | Nuclear medicine communications |
Volume | 21 |
Issue number | 7 |
State | Published - 2000 |
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging