TY - JOUR
T1 - Studies on the transport of L tyrosine into an adrenergic clone of mouse neuroblastoma
AU - Richelson, E.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1974
Y1 - 1974
N2 - Studies on the transport of L-[U 14C]tyrosine into cultured mouse neuroblastoma clone N1E 115, which has high levels of tyrosine hydroxylase (EC 1.14.16.2), are presented. This transport was by a saturable process, exhibited marked exchanging properties, and was inhibited by a reagent which attacks sulfhydryl groups but not by metabolic inhibitors. No major ion requirements were found for this transport, which was sensitive to changes in temperature but not to changes in pH in the range of 6 to 8. Molecules with close structural analogy to L tyrosine such as L 3,4 dihydroxyphenylalanine, L 3 iodotyrosine, and L phenylalanine were potent competitive inhibitors of this transport with inhibitor constants (Ki) in the range of 2 to 6 x 10-5 M. In addition, compounds such as L isoleucine, L leucine, L methionine, and L valine caused inhibition of L tyrosine transport. These results are consistent with the conclusion that L tyrosine transport into this clone is by facilitated diffusion and by a mechanism similar to the leucine preferring system which has been described by others.
AB - Studies on the transport of L-[U 14C]tyrosine into cultured mouse neuroblastoma clone N1E 115, which has high levels of tyrosine hydroxylase (EC 1.14.16.2), are presented. This transport was by a saturable process, exhibited marked exchanging properties, and was inhibited by a reagent which attacks sulfhydryl groups but not by metabolic inhibitors. No major ion requirements were found for this transport, which was sensitive to changes in temperature but not to changes in pH in the range of 6 to 8. Molecules with close structural analogy to L tyrosine such as L 3,4 dihydroxyphenylalanine, L 3 iodotyrosine, and L phenylalanine were potent competitive inhibitors of this transport with inhibitor constants (Ki) in the range of 2 to 6 x 10-5 M. In addition, compounds such as L isoleucine, L leucine, L methionine, and L valine caused inhibition of L tyrosine transport. These results are consistent with the conclusion that L tyrosine transport into this clone is by facilitated diffusion and by a mechanism similar to the leucine preferring system which has been described by others.
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M3 - Article
C2 - 4153813
AN - SCOPUS:0016201015
SN - 0021-9258
VL - 249
SP - 6218
EP - 6224
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 19
ER -