Structure-function relationships in glaucoma using enhanced depth imaging optical coherence tomography-derived parameters: A cross-sectional observational study

Flavio S. Lopes, Igor Matsubara, Izabela Almeida, Syril K. Dorairaj, Roberto M. Vessani, Augusto Paranhos Jr, Tiago S. Prata

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background: To investigate structural and functional correlations in glaucoma patients using enhanced depth imaging spectral-domain optical coherence tomography (EDI OCT)-derived parameters. Methods: We prospectively enrolled healthy participants and glaucomatous patients with a wide range of disease stages. All participants underwent visual field (VF) testing (SITA - Standard 24-2; Carl Zeiss Meditec, Dublin, CA) and EDI OCT imaging (Spectralis; Heidelberg Engineering Co., Heidelberg, Germany). The following optic nerve head parameters were measured on serial vertical EDI OCT B-scans by two experienced examiners masked to patients clinical data: lamina cribrosa (LC) thickness and area, prelaminar neural tissue thickness and area, anterior LC depth, Bruch's membrane opening (BMO) and average, superior, and inferior BMO-minimum rim width (BMO-MRW). Only good quality images were considered, and whenever both eyes were eligible, one was randomly selected for analysis. Scatter plots were constructed to investigate correlations between each anatomic parameter and patient's VF status (based on VF index [VFI] values). Results: A total of 73 eyes of 73 patients were included. All EDI OCT parameters evaluated differed significantly between glaucomatous and control eyes (P ≤ 0.045). A secondary analysis, in which glaucomatous patients were divided according to VF mean deviation index values into 3 groups (mild [G1; > - 6 dB], moderate [G2; - 6 to - 12 dB] and advanced [G3; <- 12 dB] glaucoma), revealed that average BMO-MRW was the EDI OCT parameter that presented more significant differences between the different stages of glaucoma. Significant structure-function correlations were found between VFI values and prelaminar neural tissue area (R 2 = 0.20, P = 0.017), average BMO-MRW (R 2 = 0.35, P ≤ 0.001), superior BMO-MRW (R 2 = 0.21, P = 0.012), and inferior BMO-MRW (R 2 = 0.27, P = 0.002). No significant correlations were found for LC area and anterior LC depth (P ≥ 0.452). Conclusions: Evaluating the distribution pattern and structure-function correlations of different laminar and prelaminar EDI OCT-derived parameters in glaucomatous patients, we found better results for neural tissue-based indexes (compared to LC-derived parameters). The diagnostic utility of each parameter deserves further investigations.

Original languageEnglish (US)
Article number52
JournalBMC Ophthalmology
Issue number1
StatePublished - Feb 15 2019


  • Glaucoma
  • Optic nerve diseases
  • Tomography, optical

ASJC Scopus subject areas

  • Ophthalmology


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