Stromal cell regulation of lymphoid and myeloid differentiation

P. J. Quesenberry, I. K. McNiece, B. E. Robinson, T. A. Woodward, G. B. Baber, H. E. McGrath, P. C. Isakson

Research output: Contribution to journalReview articlepeer-review

25 Scopus citations


In vitro microenvironmental influences seem to be critical for both B lymphocyte and myeloid differentiation. Studies on murine Dexter cultures and Whitlock-Witte lymphocyte cultures suggest the presence of two critical stromal regulatory cells: an alkaline-phosphatase-positive epithelioid cell and a macrophage. Further data suggest that these cells are capable of producing colony stimulating factor-1, granulocyte-macrophage CSF, a myeloid synergizing activity, and probably separate B cell growth factors. Isolation of a cell line from Dexter stroma was accomplished and this line produced CSF-1, GM-CSF, a pre-B cell and myeloid synergizing activity, and an activity acting on differentiated B cells. We speculate that the Dexter and Whitlock-Witte in vitro culture systems are regulated by factors produced by the two adherent cell types. A lineage nonspecific factor capable of inducing cells into the B lineage or synergizing with interleukin-3, GM-CSF, and CSF-1 is produced, which presumably acts on early stem cells. In addition, the cell line produces GM-CSF, CSF-1, and a factor acting on differentiated B cells. We speculte that in these culture systems, these 'terminal differentiating hormones' regulate the final pathway of differentiation, whereas the pre-B-synergizing activity supports early stem cells that can then respond to the other differentiating hormones.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalBlood Cells
Issue number1-2
StatePublished - 1987

ASJC Scopus subject areas

  • Hematology


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