Stimulation of proteoglycan synthesis with low-intensity 1 kHz vibration

Heather M. Argadine, Mark E. Bolander, James F. Greenleaf

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations


Pulsed ultrasound has become a common therapy for delayed unions and nonunions after fractures. Experiments using ATDC5 cells, a mouse clonal chondrogenic cell line, have shown that 1 kHz dynamic acoustic radiation force stimulates proteoglycan synthesis similar to 1.5 MHz pulsed ultrasound. The results of two experiments performed 8 months apart showed that chondrocytes treated with 1 kHz squarewave had a 2 to 4-fold (p<0.01) increase in total area of nodules compared to control. When the same experiments were repeated 2 months later with the same frozen cell stock, the cells were unresponsive to mechanical stimulation, suggesting the cell line had transformed and was unable to differentiate into chondrocytes. This study demonstrates the need for primary chondrocytes to further examine the biological effects of 1 kHz vibration.

Original languageEnglish (US)
Title of host publication2006 IEEE International Ultrasonics Symposium, IUS
Number of pages3
StatePublished - Dec 1 2006

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117


  • 1 kHz
  • ATDC5
  • Chondrocyte
  • Chondrogenesis

ASJC Scopus subject areas

  • Acoustics and Ultrasonics


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