Standardized flow-cytometry-based protocol to simultaneously measure transcription factor levels

Bryce A. Manso, Kay L. Medina

Research output: Contribution to journalArticlepeer-review


Transcription factor (TF) expression levels drive developmental programs, including cell fate and function, and their measurement by flow cytometry allows for robust downstream analysis. However, significant batch-to-batch variability between replicative experiments precludes direct comparison of absolute values across experimental conditions. Here, we present a flow cytometry protocol to measure the relative abundance of multiple TFs simultaneously in single cells, allowing for direct comparison across experimental conditions/time points. This protocol uses bone marrow cells but can be adapted for other cell types. For complete details on the use and execution of this protocol, please refer to Manso et al. (2021) and Manso et al. (2019).

Original languageEnglish (US)
Article number100485
JournalSTAR Protocols
Issue number2
StatePublished - Jun 18 2021


  • Cell Biology
  • Cell isolation
  • Flow Cytometry/Mass Cytometry
  • Immunology
  • Single Cell

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience


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