Species characterization of plasma nitrite/nitrate (NOx) concentration

Donna A. Williams, Debra A. Lewis, William J. Krause, Mary H. Flood, Virginia M. Miller

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Experiments were designed to detect and determine differences between nitrite/nitrate concentration ([NOx]) in plasma across 15 species selected from seven classes of vertebrates. Blood collected in syringes was placed immediately into ethylenediaminetetraacetic acid (EDTA)-containing tubes and was centrifuged. Plasma [NOx] was determined by measurement of chemiluminescence. Across classes of vertebrates, baseline plasma [NOx] ranged from 0.6 to 171.3 nmol/ml. Mean ± SD plasma [NOx] was highest in a fresh-water, jawless fish (lamprey, 95.5 ± 9.1 nmol/ml) and lowest in a saltwater cartilaginous fish (skates, 1.1 ± 0.4 nmol/ml). Both amphibians tested had a wide range in plasma [NOx], which was explained partly by temporal changes during the year. Within the mammalian class, plasma [NOx] ranged from 3.8 to 43.2 nmol/ml. Results of this study indicate that NOx is detectable in plasma of all classes of vertebrates and that baseline concentration varies among species.

Original languageEnglish (US)
Pages (from-to)21-28
Number of pages8
JournalComparative medicine
Issue number1
StatePublished - Feb 2003

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)


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