Species characterization of plasma nitrite/nitrate (NOx) concentration

Donna A. Williams; Debra A. Lewis; William J. Krause; Mary H. Flood; Virginia M. Miller

Species characterization of plasma nitrite/nitrate (NO_x) concentration

Donna A. Williams, Debra A. Lewis, William J. Krause, Mary H. Flood, Virginia M. Miller

Surgery Research

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Experiments were designed to detect and determine differences between nitrite/nitrate concentration ([NO_x]) in plasma across 15 species selected from seven classes of vertebrates. Blood collected in syringes was placed immediately into ethylenediaminetetraacetic acid (EDTA)-containing tubes and was centrifuged. Plasma [NO_x] was determined by measurement of chemiluminescence. Across classes of vertebrates, baseline plasma [NO_x] ranged from 0.6 to 171.3 nmol/ml. Mean ± SD plasma [NO_x] was highest in a fresh-water, jawless fish (lamprey, 95.5 ± 9.1 nmol/ml) and lowest in a saltwater cartilaginous fish (skates, 1.1 ± 0.4 nmol/ml). Both amphibians tested had a wide range in plasma [NO_x], which was explained partly by temporal changes during the year. Within the mammalian class, plasma [NO_x] ranged from 3.8 to 43.2 nmol/ml. Results of this study indicate that NO_x is detectable in plasma of all classes of vertebrates and that baseline concentration varies among species.

Original language	English (US)
Pages (from-to)	21-28
Number of pages	8
Journal	Comparative medicine
Volume	53
Issue number	1
State	Published - Feb 2003

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
veterinary(all)

Cite this

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title = "Species characterization of plasma nitrite/nitrate (NOx) concentration",

abstract = "Experiments were designed to detect and determine differences between nitrite/nitrate concentration ([NOx]) in plasma across 15 species selected from seven classes of vertebrates. Blood collected in syringes was placed immediately into ethylenediaminetetraacetic acid (EDTA)-containing tubes and was centrifuged. Plasma [NOx] was determined by measurement of chemiluminescence. Across classes of vertebrates, baseline plasma [NOx] ranged from 0.6 to 171.3 nmol/ml. Mean ± SD plasma [NOx] was highest in a fresh-water, jawless fish (lamprey, 95.5 ± 9.1 nmol/ml) and lowest in a saltwater cartilaginous fish (skates, 1.1 ± 0.4 nmol/ml). Both amphibians tested had a wide range in plasma [NOx], which was explained partly by temporal changes during the year. Within the mammalian class, plasma [NOx] ranged from 3.8 to 43.2 nmol/ml. Results of this study indicate that NOx is detectable in plasma of all classes of vertebrates and that baseline concentration varies among species.",

author = "Williams, {Donna A.} and Lewis, {Debra A.} and Krause, {William J.} and Flood, {Mary H.} and Miller, {Virginia M.}",

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AU - Williams, Donna A.

AU - Lewis, Debra A.

AU - Krause, William J.

AU - Flood, Mary H.

AU - Miller, Virginia M.

PY - 2003/2

Y1 - 2003/2

N2 - Experiments were designed to detect and determine differences between nitrite/nitrate concentration ([NOx]) in plasma across 15 species selected from seven classes of vertebrates. Blood collected in syringes was placed immediately into ethylenediaminetetraacetic acid (EDTA)-containing tubes and was centrifuged. Plasma [NOx] was determined by measurement of chemiluminescence. Across classes of vertebrates, baseline plasma [NOx] ranged from 0.6 to 171.3 nmol/ml. Mean ± SD plasma [NOx] was highest in a fresh-water, jawless fish (lamprey, 95.5 ± 9.1 nmol/ml) and lowest in a saltwater cartilaginous fish (skates, 1.1 ± 0.4 nmol/ml). Both amphibians tested had a wide range in plasma [NOx], which was explained partly by temporal changes during the year. Within the mammalian class, plasma [NOx] ranged from 3.8 to 43.2 nmol/ml. Results of this study indicate that NOx is detectable in plasma of all classes of vertebrates and that baseline concentration varies among species.

AB - Experiments were designed to detect and determine differences between nitrite/nitrate concentration ([NOx]) in plasma across 15 species selected from seven classes of vertebrates. Blood collected in syringes was placed immediately into ethylenediaminetetraacetic acid (EDTA)-containing tubes and was centrifuged. Plasma [NOx] was determined by measurement of chemiluminescence. Across classes of vertebrates, baseline plasma [NOx] ranged from 0.6 to 171.3 nmol/ml. Mean ± SD plasma [NOx] was highest in a fresh-water, jawless fish (lamprey, 95.5 ± 9.1 nmol/ml) and lowest in a saltwater cartilaginous fish (skates, 1.1 ± 0.4 nmol/ml). Both amphibians tested had a wide range in plasma [NOx], which was explained partly by temporal changes during the year. Within the mammalian class, plasma [NOx] ranged from 3.8 to 43.2 nmol/ml. Results of this study indicate that NOx is detectable in plasma of all classes of vertebrates and that baseline concentration varies among species.

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Species characterization of plasma nitrite/nitrate (NO_x) concentration

Abstract

ASJC Scopus subject areas

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