Abstract
Ebola virus (EBOV) uses transcriptional editing to express several glycoproteins (GPs), including secreted soluble GP (sGP) and structural GP1,2, from a single gene. Recombinant viruses predominantly expressing GP1,2 are known to rapidly mutate and acquire an editing site predominantly expressing sGP in vivo, suggesting an important role of this protein during infection. Therefore, we generated a recombinant virus that is no longer able to express sGP and assessed its virulence in the EBOV Guinea pig model. Surprisingly, although this virus remained genetically stable, it did not show any significant attenuation in vivo, showing that sGP is not required for virulence in this model.
Original language | English (US) |
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Pages (from-to) | S242-S246 |
Journal | Journal of Infectious Diseases |
Volume | 212 |
DOIs | |
State | Published - Oct 1 2015 |
Keywords
- Guinea pigs
- ebola virus
- mRNA editing
- recombinant virus
- reverse genetics
- sGP
- soluble glycoprotein
- virulence
ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases