Abstract
To quantify cell death in high-throughput studies, Gelles et al. develop a robust method for single-cell and population-level analyses using real-time kinetic labeling (SPARKL). Example protocols and mathematical analyses detail the characterization of cell death kinetics and mechanisms, with coupled changes to proliferation, for use within high-volume comparative approaches.
Original language | English (US) |
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Pages (from-to) | 277-291.e4 |
Journal | Developmental Cell |
Volume | 51 |
Issue number | 2 |
DOIs | |
State | Published - Oct 21 2019 |
Keywords
- apoptosis
- automated image analysis
- cell death
- ferroptosis
- high-throughput
- live-cell imaging
- necroptosis
- real-time assay
ASJC Scopus subject areas
- Molecular Biology
- General Biochemistry, Genetics and Molecular Biology
- Developmental Biology
- Cell Biology