Signals regulating hepatitis B surface antigen transcription

Roberto Cattaneo; Hans Will; Nouria Hernandez; Heinz Schaller

doi:10.1038/305336a0

Signals regulating hepatitis B surface antigen transcription

Roberto Cattaneo, Hans Will, Nouria Hernandez, Heinz Schaller

Molecular Medicine

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Abstract

About 200 million people are chronic carriers of hepatitis B surface antigen (HBsAg), but since hepatitis B virus (HBV) cannot be propagated in vitro, HBsAg transcription has been studied only in cell lines containing HBV DNA integrated into chromosomes, and HBsAg-related mRNAs 2.0 to 2.5 kilobases (kb) long have been described^1-4. We have analysed the transcripts produced in an infected chimpanzee liver and in a rat cell line containing HBV DNA⁵. In contrast to previous suppositions^1,2 we report here that the major S gene transcript initiates close to the S gene, that is, within the 'pre-S' region⁶ and is processed/polyadenylated at a site situated within the core gene. The efficiency of processing/polyadenylation at this site varies between the chimpanzee liver and the rat cell line studied. The S gene promoter does not contain a TATA box but instead has a sequence homologous to that which positions the 5′ ends of the major simian virus 40 (SV40) late transcript⁷.

Original language	English (US)
Pages (from-to)	336-338
Number of pages	3
Journal	Nature
Volume	305
Issue number	5932
DOIs	https://doi.org/10.1038/305336a0
State	Published - 1983

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title = "Signals regulating hepatitis B surface antigen transcription",

abstract = "About 200 million people are chronic carriers of hepatitis B surface antigen (HBsAg), but since hepatitis B virus (HBV) cannot be propagated in vitro, HBsAg transcription has been studied only in cell lines containing HBV DNA integrated into chromosomes, and HBsAg-related mRNAs 2.0 to 2.5 kilobases (kb) long have been described1-4. We have analysed the transcripts produced in an infected chimpanzee liver and in a rat cell line containing HBV DNA5. In contrast to previous suppositions1,2 we report here that the major S gene transcript initiates close to the S gene, that is, within the 'pre-S' region6 and is processed/polyadenylated at a site situated within the core gene. The efficiency of processing/polyadenylation at this site varies between the chimpanzee liver and the rat cell line studied. The S gene promoter does not contain a TATA box but instead has a sequence homologous to that which positions the 5′ ends of the major simian virus 40 (SV40) late transcript7.",

author = "Roberto Cattaneo and Hans Will and Nouria Hernandez and Heinz Schaller",

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AU - Cattaneo, Roberto

AU - Will, Hans

AU - Hernandez, Nouria

AU - Schaller, Heinz

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N2 - About 200 million people are chronic carriers of hepatitis B surface antigen (HBsAg), but since hepatitis B virus (HBV) cannot be propagated in vitro, HBsAg transcription has been studied only in cell lines containing HBV DNA integrated into chromosomes, and HBsAg-related mRNAs 2.0 to 2.5 kilobases (kb) long have been described1-4. We have analysed the transcripts produced in an infected chimpanzee liver and in a rat cell line containing HBV DNA5. In contrast to previous suppositions1,2 we report here that the major S gene transcript initiates close to the S gene, that is, within the 'pre-S' region6 and is processed/polyadenylated at a site situated within the core gene. The efficiency of processing/polyadenylation at this site varies between the chimpanzee liver and the rat cell line studied. The S gene promoter does not contain a TATA box but instead has a sequence homologous to that which positions the 5′ ends of the major simian virus 40 (SV40) late transcript7.

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Signals regulating hepatitis B surface antigen transcription

Abstract

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