Sharpening spots: Correcting for bleedover in cDNA array images

Terry Therneau, Renee C. Tschumper, Diane Jelinek

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


For cDNA array methods that depend on imaging of a radiolabel, we show that bleedover of one spot onto another, due to the gap between the array and the imaging media, can be a major problem. The images can be sharpened, however, using a blind convolution method based on the EM algorithm. The sharpened images look like a set of donuts, which concurs with our knowledge of the spotting process. Oversharpened images are actually useful as well, in locating the centers of each spot.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalMathematical Biosciences
Issue number1
StatePublished - 2002


  • EM algorithm
  • Fast Fourier transform
  • Image processing
  • cDNA arrays

ASJC Scopus subject areas

  • Statistics and Probability
  • Modeling and Simulation
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Agricultural and Biological Sciences
  • Applied Mathematics


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