Screening of bacteriophages producing endo-N-acetylneuraminidase

Katsuhide Miyake, Tsutomu Muraki, Kouji Hattori, Yuichi Machida, Masaki Watanabe, Mitsuo Kawase, Yasuko Yoshida, Shinji Iijima

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Seven different bacteriophages were isolated from sewage with Escherichia coli K1 strains as host bacteria. These phages showed specific degrading activity for the host capsular polysaccharide, α2,8-linked polysialic acid. The bacteriophage-associated endo-N-acetylneuraminidases degraded only a homopolymer of α2,8-linked sialic acid and were not able to degrade other sialylated carbohydrates such as sialyl lactose. With respect to the minimum substrate size, each enzyme had its own specificity. Among the seven phages isolated, three enzymes exhibited novel endo-N-acetylneuraminidase activity in that they produced sialyl dimer as a main product and two of them degraded sialyl trimer or tetramer.

Original languageEnglish (US)
Pages (from-to)90-93
Number of pages4
JournalJournal of Fermentation and Bioengineering
Issue number1
StatePublished - 1997


  • Bacteriophage
  • Colominic acid
  • Endo-N- acetylneuraminidase
  • Escherichia coli K1
  • Sialic acid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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