RNA-RNA noncovalent interactions investigated by microspray ionization mass spectrometry

Paula Rodrigues Hoyne, Linda M. Benson, Timothy D. Veenstra, L. James Maher, Stephen Naylor

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Electrospray ionization mass spectrometry is playing an increasing role in the study of noncovalent interactions involving biomolecules. RNA-RNA complexes are important in many areas of biology, including RNA catalysis, RNA splicing, ribosome function, and gene regulation. Here, microelectrospray mass spectrometry (μESI-MS) is used to study noncovalent base-pairing interactions between RNA oligonucleotides, an area not previously explored by this technique. Using a set of complementary RNA oligonucleotides, we demonstrate the formation of the expected double-helical RNA complexes composed of three distinct oligonucleotides. The ability to study specific RNA noncovalent interactions by μESI-MS has the potential to provide a unique method by which to analyze and assign precise molecular masses to RNA-RNA complexes.

Original languageEnglish (US)
Pages (from-to)1539-1547
Number of pages9
JournalRapid Communications in Mass Spectrometry
Issue number17
StatePublished - 2001

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry


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