Reproducibility of 3D micro-CT gray-scale and structural dimension data in longitudinal studies

Steven M. Jorgensen, Diane R. Eaker, Andrew J. Vercnocke, Erik L. Ritman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations


Repeated micro-CT scanning of a number of iliac crest biopsies enabled us to quantitate the variation in CT image gray-scale and spatial geometry due to variables such as specimen orientation, projection magnification, voxel size and slight differences in x-ray photon energy in each of the different scans. Using the micro-CT scanner on beamline X2B at the Brookhaven National Laboratory's National Synchrotron Light Source, we rescanned several iliac crest bone biopsy specimens, and a test phantom made of calcium hydroxyapatite, at repeated scanning sessions and evaluated the reproducibility of the spatial geometry and gray-scale characteristics of the specimens. This scanner consists of a Bragg diffraction source of monochromatic x-rays, a computer controlled high precision specimen rotation and translation stage assembly, and a fluorescent crystal and CCD array system for imaging the specimen at each of the angles of view around its axis of rotation during the scanning sequence. The 3-D micro-CT images consisted of up to 1024×24002, 4 μm, cubic voxels, each with 16-bit gray-scale. We also reconstructed the images at 16, 32 and 48 μm voxel resolution. Partial volume effects at the surface of the bone were diminished by 'eroding' the surface voxels in the 4 μm images, but significantly changed the outcome at greater voxel size. Reproducibility of the mineral content of bone, at mean bone opacity value, was ± 28.8 mg/cm3, i.e., 2.56%, in a 4 μm cubic voxel at the 95% confidence level.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2006
Subtitle of host publicationPhysiology, Function, and Structure from Medical Images
StatePublished - 2006
EventMedical Imaging 2006: Physiology, Function, and Structure from Medical Images - San Diego, CA, United States
Duration: Feb 12 2006Feb 14 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6143 II
ISSN (Print)1605-7422


OtherMedical Imaging 2006: Physiology, Function, and Structure from Medical Images
Country/TerritoryUnited States
CitySan Diego, CA


  • Bone
  • Interpolation
  • Mineralization
  • Partial volume effects
  • Photon energy bandwidth
  • Voxel size
  • X-ray synchrotron

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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