Abstract
Intra-acinar cell nuclear factor-κB (NF-κB) and trypsinogen activation are early events in secretagogue-induced acute pancreatitis. We have studied the relationship between NF-κB and trypsinogen activation in rat pancreas. CCK analogue caerulein induces early (within 15 rain) parallel activation of both NF-κB and trypsinogen in pancreas in viva as well as in pancreatic acini in vitro. However, NF-κB activation can be induced without trypsinogen activation by lipopolysaccharide in pancreas in viva and by phorbol ester in pancreatic acini in vitro. Stimulation of acini with caerulein after 6 h of culture results in NF-κB but not trypsinogen activation. Protease inhibitors (AEBSF, TLCK, and E64d) inhibit both intracellular trypsin activity and NF-κB activation in caerulein stimulated acini. A chymotrypsin inhibitor (TPCK) inhibits NF-κB activation but not trypsin activity. The proteasome inhibitor MG-132 prevents caerulein-induced NF-κB activation but does not prevent trypsinogen activation. These findings indicate that although caerulein-induced NF-κB and trypsinogen activation are temporally closely related, they are independent events in pancreatic acinar cells. NF-κB activation per se is not required for the development of early acinar cell injury by supramaximal secretagogue stimulation.
Original language | English (US) |
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Pages (from-to) | 388-395 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 280 |
Issue number | 1 |
DOIs | |
State | Published - 2001 |
Keywords
- Acinar cell injury
- Caerulein
- Digestive enzymes
- Nuclear factor-κB
- Pancreas
- Transcription factor
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology