Relationship between FRA11F and 11q13 gene amplification in oral cancer

Shalini C. Reshmi, Xin Huang, David W. Schoppy, Robert C. Black, William S. Saunders, David I. Smith, Susanne M. Gollin

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Common fragile sites (CFS) are nonstaining gaps or breaks in chromosomes that are expressed under conditions inducing replicative stress. CFS have been suggested to play a role in epithelial cancers by their association with loss of heterozygosity, loss of gene expression, and/or gene amplification in the form of homogeneously staining regions (hsrs). In oral squamous-cell carcinomas (OSCC), amplification of chromosomal band 11q13 occurs in the form of an hsr. We suggested previously that CFS flanking 11q13 may be susceptible to breakage induced by tobacco or other carcinogens and/or human papillomavirus, promoting formation of the 11q13 amplicon. Examination of OSCC cell lines with 11q13 amplification using fluorescence in situ hybridization showed loss of FRA11F sequences, whereas cell lines without 11q13 amplification displayed an intact FRA11F site. Cell lines with more complex 11q rearrangements expressed FRA11F in the form of an inverted duplication, characteristic of breakage-fusion-bridge cycles. Our findings suggest that gene amplification involving chromosomal band 11q13 in OSCC may be initiated by breakage at FRA11F.

Original languageEnglish (US)
Pages (from-to)143-154
Number of pages12
JournalGenes Chromosomes and Cancer
Issue number2
StatePublished - Feb 2007

ASJC Scopus subject areas

  • Genetics
  • Cancer Research


Dive into the research topics of 'Relationship between FRA11F and 11q13 gene amplification in oral cancer'. Together they form a unique fingerprint.

Cite this