Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing

J. M. England; R. M. Rowan; O. W. Van Assendelft; B. S. Bull; W. Coulter; K. Fujimoto; W. Groner; A. Richardson-Jones; G. Klee; J. A. Koepke; S. M. Lewis; C. E. McLaren; N. K. Shinton; N. Tatsumi; R. L. Verwilghen

doi:10.1093/ajcp/100.4.371

Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing

J. M. England, R. M. Rowan, O. W. Van Assendelft, B. S. Bull, W. Coulter, K. Fujimoto, W. Groner, A. Richardson-Jones, G. Klee, J. A. Koepke, S. M. Lewis, C. E. McLaren, N. K. Shinton, N. Tatsumi, R. L. Verwilghen

Laboratory Medicine and Pathology

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99 Scopus citations

Abstract

Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt. Therefore, dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing. The amount of dipotassium EDTA used is 1.5-2.2 mg (3.7-5.4 μmol) per milliliter of blood.

Original language	English (US)
Pages (from-to)	371-372
Number of pages	2
Journal	American journal of clinical pathology
Volume	100
Issue number	4
DOIs	https://doi.org/10.1093/ajcp/100.4.371
State	Published - 1993

Keywords

Anticoagulant
Blood cell count
EDTA

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1093/ajcp/100.4.371

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England, J. M., Rowan, R. M., Van Assendelft, O. W., Bull, B. S., Coulter, W., Fujimoto, K., Groner, W., Richardson-Jones, A., Klee, G., Koepke, J. A., Lewis, S. M., McLaren, C. E., Shinton, N. K., Tatsumi, N., & Verwilghen, R. L. (1993). Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing. American journal of clinical pathology, 100(4), 371-372. https://doi.org/10.1093/ajcp/100.4.371

Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing. / England, J. M.; Rowan, R. M.; Van Assendelft, O. W. et al.
In: American journal of clinical pathology, Vol. 100, No. 4, 1993, p. 371-372.

Research output: Contribution to journal › Article › peer-review

England, JM, Rowan, RM, Van Assendelft, OW, Bull, BS, Coulter, W, Fujimoto, K, Groner, W, Richardson-Jones, A, Klee, G, Koepke, JA, Lewis, SM, McLaren, CE, Shinton, NK, Tatsumi, N & Verwilghen, RL 1993, 'Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing', American journal of clinical pathology, vol. 100, no. 4, pp. 371-372. https://doi.org/10.1093/ajcp/100.4.371

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abstract = "Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt. Therefore, dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing. The amount of dipotassium EDTA used is 1.5-2.2 mg (3.7-5.4 μmol) per milliliter of blood.",

keywords = "Anticoagulant, Blood cell count, EDTA",

author = "England, {J. M.} and Rowan, {R. M.} and {Van Assendelft}, {O. W.} and Bull, {B. S.} and W. Coulter and K. Fujimoto and W. Groner and A. Richardson-Jones and G. Klee and Koepke, {J. A.} and Lewis, {S. M.} and McLaren, {C. E.} and Shinton, {N. K.} and N. Tatsumi and Verwilghen, {R. L.}",

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AU - Van Assendelft, O. W.

AU - Bull, B. S.

AU - Coulter, W.

AU - Fujimoto, K.

AU - Groner, W.

AU - Richardson-Jones, A.

AU - Klee, G.

AU - Koepke, J. A.

AU - Lewis, S. M.

AU - McLaren, C. E.

AU - Shinton, N. K.

AU - Tatsumi, N.

AU - Verwilghen, R. L.

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AB - Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt. Therefore, dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing. The amount of dipotassium EDTA used is 1.5-2.2 mg (3.7-5.4 μmol) per milliliter of blood.

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Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing

Abstract

Keywords

ASJC Scopus subject areas

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