Recommendations for reference method for the packed cell volume (ICSH Standard 2001)

O. W. Van Assendelft, B. S. Bull, K. Fujimoto, W. Groner, B. Houwen, L. Van Hove, G. G. Klee, J. A. Koepke, S. M. Lewis, G. D'Onofrio, R. M. Rowan, N. Tatsumi, C. E. McLaren

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The manual, spun Packed Cell Volume (PCV, hematocrit) is a key measurement on which are based hematology instrument calibration, reference range determination and assignment of values to calibrators/controls. Until now, however, no true reference method for the PCV has existed. The International Council for Standardisation in Haematology (ICSH) which met in Montpelier, France in May 2001 has now recommended a reference method for the PCV. This reference PCV method is fully traceable to the ICSH reference method for haemoglobinometry. Using carefully defined capillary tubes, the reference PCV can be calculated as the ratio between the hemoglobin of whole blood and the hemoglobin of packed red blood cells with all plasma removed. The ICSH intends that this reference protocol be used primarily by manufacturers of capillary microhematocrit tubes, liquid calibrators and multichannel analyzers.

Original languageEnglish (US)
Pages (from-to)148-170
Number of pages23
JournalLaboratory Hematology
Issue number3
StatePublished - 2001


  • Hematocrit
  • Packed cell volume (PCV)
  • Reference method

ASJC Scopus subject areas

  • Hematology
  • Clinical Biochemistry
  • Biochemistry, medical


Dive into the research topics of 'Recommendations for reference method for the packed cell volume (ICSH Standard 2001)'. Together they form a unique fingerprint.

Cite this