Rapid downregulation of NCX and PMCA in hippocampal neurons following H2O2 oxidative stress

Sertac N. Kip, Emanuel E. Strehler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

23 Scopus citations


Na+/Ca2+ exchangers (NCXs) and plasma membrane Ca2+ pumps (PMCAs) are crucial for intracellular Ca2+ homeostasis and Ca2+ signaling. Elevated [Ca2+] i is a hallmark of neurodegenerative disease and stroke. Here we studied the short-term effect of oxidative stress on the plasma membrane Ca 2+ extrusion systems in hippocampal neurons (HN) and found that after 2-3 h exposure to 300 μM H2O2, all NCXs and PMCAs were significantly downregulated at the RNA (NCX) and protein (PMCA) level. Rapid internalization and aggregation of the PMCA was also observed. Our data show that the plasma membrane calcium extrusion systems are sensitive early targets of neurotoxic oxidative stress.

Original languageEnglish (US)
Title of host publicationSodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion
Subtitle of host publicationFifth International Conference
PublisherBlackwell Publishing Inc.
Number of pages4
ISBN (Print)1573316490, 9781573316491
StatePublished - Mar 2007

Publication series

NameAnnals of the New York Academy of Sciences
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632


  • Calcium extrusion
  • Hippocampal neurons
  • NCX
  • Na/Ca exchanger
  • Oxidative stress
  • PMCA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science


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