TY - JOUR
T1 - Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue
AU - Yuk, Hyunwoo
AU - Wu, Jingjing
AU - Sarrafian, Tiffany L.
AU - Mao, Xinyu
AU - Varela, Claudia E.
AU - Roche, Ellen T.
AU - Griffiths, Leigh G.
AU - Nabzdyk, Christoph S.
AU - Zhao, Xuanhe
N1 - Funding Information:
We thank the Koch Institute Swanson Biotechnology Center for technical support, specifically K. Cormier and the Histology Core for the histological processing, and R. Bronson at Harvard Medical School for the histological evaluations. This work is supported by the MIT Deshpande Center (H.Y., C.S.N., X.Z.), National Institutes of Health (1-R01-HL153857-01, X.Z.), National Science Foundation (EFMA-1935291, X.Z.), the US Army Research Office through the Institute for Soldier Nanotechnologies at MIT (W911NF-13-D-0001) and the ZOLL Foundation (C.S.N.). H.Y. acknowledges financial support from Samsung Scholarship.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2021/10
Y1 - 2021/10
N2 - Tissue adhesives do not normally perform well on tissues that are covered with blood or other bodily fluids. Here we report the design, adhesion mechanism and performance of a paste that haemostatically seals tissues in less than 15 s, independently of the blood-coagulation rate. With a design inspired by barnacle glue (which strongly adheres to wet and contaminated surfaces owing to adhesive proteins embedded in a lipid-rich matrix), the paste consists of a blood-repelling hydrophobic oil matrix containing embedded microparticles that covalently crosslink with tissue surfaces on the application of gentle pressure. It slowly resorbs over weeks, sustains large pressures (approximately 350 mm Hg of burst pressure in a sealed porcine aorta), makes tough (interfacial toughness of 150–300 J m−2) and strong (shear and tensile strengths of, respectively, 40–70 kPa and 30–50 kPa) interfaces with blood-covered tissues, and outperforms commercial haemostatic agents in the sealing of bleeding porcine aortas ex vivo and of bleeding heart and liver tissues in live rats and pigs. The paste may aid the treatment of severe bleeding, even in individuals with coagulopathies.
AB - Tissue adhesives do not normally perform well on tissues that are covered with blood or other bodily fluids. Here we report the design, adhesion mechanism and performance of a paste that haemostatically seals tissues in less than 15 s, independently of the blood-coagulation rate. With a design inspired by barnacle glue (which strongly adheres to wet and contaminated surfaces owing to adhesive proteins embedded in a lipid-rich matrix), the paste consists of a blood-repelling hydrophobic oil matrix containing embedded microparticles that covalently crosslink with tissue surfaces on the application of gentle pressure. It slowly resorbs over weeks, sustains large pressures (approximately 350 mm Hg of burst pressure in a sealed porcine aorta), makes tough (interfacial toughness of 150–300 J m−2) and strong (shear and tensile strengths of, respectively, 40–70 kPa and 30–50 kPa) interfaces with blood-covered tissues, and outperforms commercial haemostatic agents in the sealing of bleeding porcine aortas ex vivo and of bleeding heart and liver tissues in live rats and pigs. The paste may aid the treatment of severe bleeding, even in individuals with coagulopathies.
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U2 - 10.1038/s41551-021-00769-y
DO - 10.1038/s41551-021-00769-y
M3 - Article
C2 - 34373600
AN - SCOPUS:85112199205
SN - 2157-846X
VL - 5
SP - 1131
EP - 1142
JO - Nature Biomedical Engineering
JF - Nature Biomedical Engineering
IS - 10
ER -