@article{22f29c4569a346dbabb8f617dd3c9759,
title = "Radiation Triggers a Dynamic Sequence of Transient Microglial Alterations in Juvenile Brain",
abstract = "Osman et al. show that microglia undergo transient changes after radiation, displaying a series of temporally distinct molecular signatures with a trajectory toward baseline within one week and suggesting a narrow time window for any therapeutic interventions specifically targeting early microglial activation after irradiation.",
keywords = "M1 and M2, RNA sequencing, neuroinflammation, radiotherapy, transcriptome",
author = "Osman, {Ahmed M.} and Ying Sun and Burns, {Terry C.} and Liqun He and Nigel Kee and Nuria Oliva-Vilarnau and Androniki Alevyzaki and Kai Zhou and Lauri Louhivuori and Per Uhl{\'e}n and Eva Hedlund and Christer Betsholtz and Lauschke, {Volker M.} and Julianna Kele and Klas Blomgren",
note = "Funding Information: This work was supported by the Swedish Childhood Cancer Fund , the Swedish Cancer Foundation , the Swedish Research Council , governmental grants provided by the Stockholm County Council (ALF projects), the Frimurare Barnhus Foundation in Stockholm, the KI Foundation for Research , the M{\"a}rta and Gunnar V Philipson Foundation , the Swedish Brain Foundation , and the Swedish Board of Radiation Safety . J.K. appreciatively acknowledges support from the Jane and Dan Olsson Foundations , the Lars Hierta Memorial Foundation , Tore Nilsons Stiftelse f{\"o}r Medicinsk Forskning, the Foundation Tornspiran, the {\AA}ke Wiberg Foundation, O.E. och Edla Johanssons Vetenskapliga Stiftelse, and the Olle Engkvist Foundation . T.C.B. gratefully acknowledges support from Regenerative Medicine Minnesota , NIH NS109770 , Humor To Fight The Tumo r, and L.B. & Terrie McKelvey . We also thank Javier Avila-Cari{\~n}o at the fluorescence-activated cell sorting (FACS) facility at the Department of Cell and Molecular Biology (CMB), Karolinska Institutet, for assisting with the cell sorting; the core facility Bioinformatics and Expression Analysis (BEA, Karolinska Institute, supported by the Board of Research at the Karolinska Institutet and the Research Committee at the Karolinska University Hospital) for help with the bulk RNA-seq and special thanks to David Brodin for his supportive assistance; and the Eukaryotic Single Cell Genomics Facility (ESCG, Karolinska Institute) for running the single-cell RNA-seq. Funding Information: This work was supported by the Swedish Childhood Cancer Fund, the Swedish Cancer Foundation, the Swedish Research Council, governmental grants provided by the Stockholm County Council (ALF projects), the Frimurare Barnhus Foundation in Stockholm, the KI Foundation for Research, the M?rta and Gunnar V Philipson Foundation, the Swedish Brain Foundation, and the Swedish Board of Radiation Safety. J.K. appreciatively acknowledges support from the Jane and Dan Olsson Foundations, the Lars Hierta Memorial Foundation, Tore Nilsons Stiftelse f?r Medicinsk Forskning, the Foundation Tornspiran, the ?ke Wiberg Foundation, O.E. och Edla Johanssons Vetenskapliga Stiftelse, and the Olle Engkvist Foundation. T.C.B. gratefully acknowledges support from Regenerative Medicine Minnesota, NIH NS109770, Humor To Fight The Tumor, and L.B. & Terrie McKelvey. We also thank Javier Avila-Cari?o at the fluorescence-activated cell sorting (FACS) facility at the Department of Cell and Molecular Biology (CMB), Karolinska Institutet, for assisting with the cell sorting; the core facility Bioinformatics and Expression Analysis (BEA, Karolinska Institute, supported by the Board of Research at the Karolinska Institutet and the Research Committee at the Karolinska University Hospital) for help with the bulk RNA-seq and special thanks to David Brodin for his supportive assistance; and the Eukaryotic Single Cell Genomics Facility (ESCG, Karolinska Institute) for running the single-cell RNA-seq. A.M.O. performed the animal experiments, histological analysis, and isolation of MG. K.Z. and A.A. contributed to the histological analysis. N.O.-V. and V.M.L. analyzed the bulk RNA-seq data. Y.S. L.H. C.B. N.K. and E.H. analyzed the single-cell RNA-seq data. T.C.B. performed GSEA. L.L. and P.U. contributed to the in vivo imaging. A.M.O. J.K. and K.B. designed the study, interpreted the results, and wrote the manuscript. All authors discussed the results and commented on or edited the manuscript. V.M.L. is co-founder and owner of HepaPredict AB. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = jun,
day = "2",
doi = "10.1016/j.celrep.2020.107699",
language = "English (US)",
volume = "31",
journal = "Cell reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "9",
}