TY - JOUR
T1 - Quantitative method for detection of blood-nerve barrier alterations in experimental animal models of neuropathy
AU - Ohi, Takekazu
AU - Poduslo, Joseph F.
AU - Curran, Geoffry L.
AU - Dyck, Peter J.
N1 - Funding Information:
Abbreviations: BNB-blood-nerve barrier, RIA-radioimmunoassay. ’ This investigation was supported by a Peripheral Neuropathy Clinical Center Grant from the National Institutes of Health (NSl4304) and the Borchard Fund. We thank Alfred C. Lais and Carole T. Berg for technical assistance and Anita D. Payne for secretarial assistance. Please address correspondence and reprint requests to Dr. Joseph F. Poduslo.
PY - 1985/11
Y1 - 1985/11
N2 - A solid phase radioimmunoassay was developed for measuring albumin concentrations in both endoneurium and serum, which were normalized to total endoneurium and serum protein to obtain a blood-nerve barrier index (BNB-index). The BNB-index in experimental lead neuropathy demonstrated barrier dysfunction beginning at 6 weeks of 4% lead carbonate ingestion and at 14 weeks was 5.2 times that of pair-fed controls. These data, therefore, confirmed investigations that indicated a gradual alteration of the BNB beginning at 6 weeks and were based on (i) direct measurement of endoneurial albumin concentration by densitometry after sodium dodecyl sulfate-pore gradient electrophoresis and (ii) intravenous injection of 125I-albumin (J. F. Poduslo, P. A. Low, A. J. Windebank, P. J. Dyck, C. T. Berg, and J. D. Schmeltzer, 1982.J. Neurosci.2: 1507-1514). The BNB-index after crush injury was 2.2 times that of control nerves at 24 h and gradually decreased toward normal values but was still 1.6 times that of controls at 70 days, a value consistent with the prolonged time course for complete repair. The BNB-index, therefore, can be used to evaluate BNB alterations quantitatively in animal models of neuropathy. Furthermore, we suggest that the BNB-index can also be used on biopsied, neuropathic, human sural nerve for evaluation of blood-nerve barrier abnormality.
AB - A solid phase radioimmunoassay was developed for measuring albumin concentrations in both endoneurium and serum, which were normalized to total endoneurium and serum protein to obtain a blood-nerve barrier index (BNB-index). The BNB-index in experimental lead neuropathy demonstrated barrier dysfunction beginning at 6 weeks of 4% lead carbonate ingestion and at 14 weeks was 5.2 times that of pair-fed controls. These data, therefore, confirmed investigations that indicated a gradual alteration of the BNB beginning at 6 weeks and were based on (i) direct measurement of endoneurial albumin concentration by densitometry after sodium dodecyl sulfate-pore gradient electrophoresis and (ii) intravenous injection of 125I-albumin (J. F. Poduslo, P. A. Low, A. J. Windebank, P. J. Dyck, C. T. Berg, and J. D. Schmeltzer, 1982.J. Neurosci.2: 1507-1514). The BNB-index after crush injury was 2.2 times that of control nerves at 24 h and gradually decreased toward normal values but was still 1.6 times that of controls at 70 days, a value consistent with the prolonged time course for complete repair. The BNB-index, therefore, can be used to evaluate BNB alterations quantitatively in animal models of neuropathy. Furthermore, we suggest that the BNB-index can also be used on biopsied, neuropathic, human sural nerve for evaluation of blood-nerve barrier abnormality.
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U2 - 10.1016/0014-4886(85)90025-1
DO - 10.1016/0014-4886(85)90025-1
M3 - Article
C2 - 2996925
AN - SCOPUS:0022368265
SN - 0014-4886
VL - 90
SP - 365
EP - 372
JO - Experimental Neurology
JF - Experimental Neurology
IS - 2
ER -