Quantitative analysis of influenza virus-specific B cell memory generated by different routes of inactivated virus vaccination

Hye Mee Joo; Yuxia He; Aarthi Sundararajan; Lifang Huan; Mark Y. Sangster

doi:10.1016/j.vaccine.2009.12.058

Quantitative analysis of influenza virus-specific B cell memory generated by different routes of inactivated virus vaccination

Hye Mee Joo, Yuxia He, Aarthi Sundararajan, Lifang Huan, Mark Y. Sangster

Allergy, Asthma and Clinical Immunology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We consider both Ab-secreting cell (ASC) and memory B cell (B_Mem) populations in a quantitative analysis of virus-specific B cell memory generated by intramuscular or intranasal vaccination of mice with inactivated influenza virus. After both forms of vaccination, the memory phase was characterized by localization of ASCs in the bone marrow and dispersion of B_Mem to organized lymphoid tissues. The stronger IgG response to intramuscular vaccination correlated with larger numbers of IgG ASCs in the bone marrow and IgG B_Mem. IgA production was only prominent in the response to intranasal vaccination and was associated with IgA ASC localization in the lung and IgA B_Mem formation. Notably, few IgG ASCs or B_Mem localized in the lung after intramuscular vaccination, in contrast to the situation following influenza pneumonia. Our analysis links the nature of immunization to characteristics of the state of B cell memory that may relate to protective immunity.

Original language	English (US)
Pages (from-to)	2186-2194
Number of pages	9
Journal	Vaccine
Volume	28
Issue number	10
DOIs	https://doi.org/10.1016/j.vaccine.2009.12.058
State	Published - Mar 2 2010

Keywords

Antibodies
B cell memory
Influenza vaccination

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2009.12.058

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title = "Quantitative analysis of influenza virus-specific B cell memory generated by different routes of inactivated virus vaccination",

abstract = "We consider both Ab-secreting cell (ASC) and memory B cell (BMem) populations in a quantitative analysis of virus-specific B cell memory generated by intramuscular or intranasal vaccination of mice with inactivated influenza virus. After both forms of vaccination, the memory phase was characterized by localization of ASCs in the bone marrow and dispersion of BMem to organized lymphoid tissues. The stronger IgG response to intramuscular vaccination correlated with larger numbers of IgG ASCs in the bone marrow and IgG BMem. IgA production was only prominent in the response to intranasal vaccination and was associated with IgA ASC localization in the lung and IgA BMem formation. Notably, few IgG ASCs or BMem localized in the lung after intramuscular vaccination, in contrast to the situation following influenza pneumonia. Our analysis links the nature of immunization to characteristics of the state of B cell memory that may relate to protective immunity.",

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author = "Joo, {Hye Mee} and Yuxia He and Aarthi Sundararajan and Lifang Huan and Sangster, {Mark Y.}",

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AU - He, Yuxia

AU - Sundararajan, Aarthi

AU - Huan, Lifang

AU - Sangster, Mark Y.

N1 - Funding Information: This research was supported by National Institutes of Health Grant AI061709 (to M.Y.S.).

PY - 2010/3/2

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AB - We consider both Ab-secreting cell (ASC) and memory B cell (BMem) populations in a quantitative analysis of virus-specific B cell memory generated by intramuscular or intranasal vaccination of mice with inactivated influenza virus. After both forms of vaccination, the memory phase was characterized by localization of ASCs in the bone marrow and dispersion of BMem to organized lymphoid tissues. The stronger IgG response to intramuscular vaccination correlated with larger numbers of IgG ASCs in the bone marrow and IgG BMem. IgA production was only prominent in the response to intranasal vaccination and was associated with IgA ASC localization in the lung and IgA BMem formation. Notably, few IgG ASCs or BMem localized in the lung after intramuscular vaccination, in contrast to the situation following influenza pneumonia. Our analysis links the nature of immunization to characteristics of the state of B cell memory that may relate to protective immunity.

KW - Antibodies

KW - B cell memory

KW - Influenza vaccination

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Quantitative analysis of influenza virus-specific B cell memory generated by different routes of inactivated virus vaccination

Abstract

Keywords

ASJC Scopus subject areas

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