Quantification of Muscle Stem Cell Differentiation Using Live-Cell Imaging and Eccentricity Measures

Paige C. Arneson-Wissink, Jason D. Doles

Research output: Chapter in Book/Report/Conference proceedingChapter


Culturing primary muscle stem cells ex vivo is a useful method for studying this cell population in controlled environments. Primary muscle stem cells respond to external stimuli differently than immortalized myoblasts (C2C12 cells), making ex vivo culture of muscle stem cells an important tool in understanding cell responses to stimuli. Primary muscle stem cells cultured ex vivo retain a majority of the characteristics they possess in vivo such as the abilities to differentiate into multinucleated structures, and self-renew a stem cell-like population. In this chapter, we describe methods for isolating primary muscle stem cells, controlled differentiation into myotubes, and quantification of differentiation using IncuCyte live cell imaging and analysis software.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Differentiation
  • Eccentricity
  • IncuCyte ZOOM
  • Muscle stem cells
  • Myotubes
  • Satellite cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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