Abstract
Multicellular spheroids are useful models for drug testing or studying tumor biology, but their production requires specialized approaches. Here, we present a protocol to produce viable spheroids by slow rotation around a horizontal axis using standard culture tubes. We describe steps for both seed and starter culture, and maintenance and expansion of spheroids. We detail assessment of spheroid size, count, viability, and immunohistochemistry. This protocol reduces gravitational forces that lead to cell clumping and is amenable to high-throughput use.
Original language | English (US) |
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Article number | 102264 |
Journal | STAR Protocols |
Volume | 4 |
Issue number | 2 |
DOIs | |
State | Published - Jun 16 2023 |
Keywords
- Cancer
- Cell Biology
- Cell culture
- Cell-based Assays
- Organoids
ASJC Scopus subject areas
- General Neuroscience
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology