@inbook{3458147cd4f44fac97cc3e2f8c16f3c6,
title = "Proteomic strategies to characterize signaling pathways",
abstract = "Cells respond to external stimuli by transducing signals through a series of intracellular molecules and eliciting an appropriate response. The cascade of events through which the signals are transduced include post-translational modifications such as phosphorylation and ubiquitylation in addition to formation of multi-protein complexes. Improvements in biological mass spectrometry and protein/peptide microarray technology have tremendously improved our ability to probe proteins, protein complexes, and signaling pathways in a high-throughput fashion. Today, a single mass spectrometry-based investigation of a signaling pathway has the potential to uncover the large majority of known signaling intermediates painstakingly characterized over decades in addition to discovering a number of novel ones. Here, we discuss various proteomic strategies to characterize signaling pathways and provide protocols for phosphoproteomic analysis.",
keywords = "Cancer, Enrichment of phosphoproteomes, Mass spectrometry, Phosphorylation, Quantitative phosphoproteomics, Signaling pathways",
author = "Harsha, {H. C.} and Pinto, {Sneha M.} and Akhilesh Pandey",
year = "2013",
doi = "10.1007/978-1-62703-392-3_16",
language = "English (US)",
isbn = "9781627033916",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "359--377",
booktitle = "Mass Spectrometry Data Analysis in Proteomics",
}