Proteomic strategies to characterize signaling pathways

H. C. Harsha, Sneha M. Pinto, Akhilesh Pandey

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations


Cells respond to external stimuli by transducing signals through a series of intracellular molecules and eliciting an appropriate response. The cascade of events through which the signals are transduced include post-translational modifications such as phosphorylation and ubiquitylation in addition to formation of multi-protein complexes. Improvements in biological mass spectrometry and protein/peptide microarray technology have tremendously improved our ability to probe proteins, protein complexes, and signaling pathways in a high-throughput fashion. Today, a single mass spectrometry-based investigation of a signaling pathway has the potential to uncover the large majority of known signaling intermediates painstakingly characterized over decades in addition to discovering a number of novel ones. Here, we discuss various proteomic strategies to characterize signaling pathways and provide protocols for phosphoproteomic analysis.

Original languageEnglish (US)
Title of host publicationMass Spectrometry Data Analysis in Proteomics
PublisherHumana Press Inc.
Number of pages19
ISBN (Print)9781627033916
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cancer
  • Enrichment of phosphoproteomes
  • Mass spectrometry
  • Phosphorylation
  • Quantitative phosphoproteomics
  • Signaling pathways

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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