Abstract
The present study investigated whether Shenqi-wan possesses a protective effect against hydrogen peroxide (H2O2)-induced apoptosis of the hippocampal cell line HiB5. Through morphological and biochemical analyses, it was demonstrated that HiB5 cells treated with H2O 2 exhibited several apoptotic features, while cells pre-treated with Shenqi-wan prior to H2O2 exposure showed a decrease in the occurrence of apoptosis. In addition, a patch clamp study revealed that Shenqi-wan inhibited profoundly N-methyl-D-aspartic acid (NMDA) receptor-activated ion current in acutely dissociated hippocampal CA1 neurons. These results suggest that Shenqi-wan may exert its protective effect against H2O2-induced apoptosis via inhibition of NMDA receptors in hippocampal neuronal cells.
Original language | English (US) |
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Pages (from-to) | 675-686 |
Number of pages | 12 |
Journal | American Journal of Chinese Medicine |
Volume | 31 |
Issue number | 5 |
DOIs | |
State | Published - 2003 |
Keywords
- Corni fructus
- Hippocampus
- Hydrogen peroxide
- N-methyl-D-aspartic acid receptor
- Rehmanniae radix
- Shenqi-wan
ASJC Scopus subject areas
- Complementary and alternative medicine