Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML- 1/CBF-α2

Lei Tang; Bo Guo; Andre J. Van Wijnen; Jane B. Lian; Janet L. Stein; Gary S. Stein; G. Wayne Zhou

doi:10.1006/jsbi.1998.4016

Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML- 1/CBF-α2

Lei Tang, Bo Guo, Andre J. Van Wijnen, Jane B. Lian, Janet L. Stein, Gary S. Stein, G. Wayne Zhou

Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-α2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-α2 protein to the nuclear matrix. The crystal belongs to tetragonal space group P4₃2₁2 with unit cell dimensions a = b = 93.4 Å, c = 57.6 Å. There is one GST- fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 Å and are appropriate for structure determination.

Original language	English (US)
Pages (from-to)	83-85
Number of pages	3
Journal	Journal of Structural Biology
Volume	123
Issue number	1
DOIs	https://doi.org/10.1006/jsbi.1998.4016
State	Published - Sep 1998

Keywords

Leukemia
Nuclear matrix
X-ray diffraction

ASJC Scopus subject areas

Structural Biology

Access to Document

10.1006/jsbi.1998.4016

Cite this

@article{df3260a04b2a4686a358e5c69eb2e481,

title = "Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML- 1/CBF-α2",

abstract = "A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-α2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-α2 protein to the nuclear matrix. The crystal belongs to tetragonal space group P43212 with unit cell dimensions a = b = 93.4 {\AA}, c = 57.6 {\AA}. There is one GST- fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 {\AA} and are appropriate for structure determination.",

keywords = "Leukemia, Nuclear matrix, X-ray diffraction",

author = "Lei Tang and Bo Guo and {Van Wijnen}, {Andre J.} and Lian, {Jane B.} and Stein, {Janet L.} and Stein, {Gary S.} and Zhou, {G. Wayne}",

note = "Funding Information: This work was supported by a Career Development Award from the American Diabetes Association and by Grant AR 45688 from the National Institutes of Health.",

year = "1998",

month = sep,

doi = "10.1006/jsbi.1998.4016",

language = "English (US)",

volume = "123",

pages = "83--85",

journal = "Journal of Structural Biology",

issn = "1047-8477",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML- 1/CBF-α2

AU - Tang, Lei

AU - Guo, Bo

AU - Van Wijnen, Andre J.

AU - Lian, Jane B.

AU - Stein, Janet L.

AU - Stein, Gary S.

AU - Zhou, G. Wayne

N1 - Funding Information: This work was supported by a Career Development Award from the American Diabetes Association and by Grant AR 45688 from the National Institutes of Health.

PY - 1998/9

Y1 - 1998/9

N2 - A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-α2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-α2 protein to the nuclear matrix. The crystal belongs to tetragonal space group P43212 with unit cell dimensions a = b = 93.4 Å, c = 57.6 Å. There is one GST- fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 Å and are appropriate for structure determination.

AB - A glutathione S-transferase fused with the nuclear matrix targeting signal (GST-NMTS) of AML-1/CBF-α2 has been crystallized by the vapor diffusion method using polyethylene glycol (PEG) as the precipitant. The NMTS is a 31-amino-acid signal peptide that can target the AML-1/CBF-α2 protein to the nuclear matrix. The crystal belongs to tetragonal space group P43212 with unit cell dimensions a = b = 93.4 Å, c = 57.6 Å. There is one GST- fusion protein per asymmetric unit. Crystals diffracted to at least 2.7 Å and are appropriate for structure determination.

KW - Leukemia

KW - Nuclear matrix

KW - X-ray diffraction

UR - http://www.scopus.com/inward/record.url?scp=0032170325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032170325&partnerID=8YFLogxK

U2 - 10.1006/jsbi.1998.4016

DO - 10.1006/jsbi.1998.4016

M3 - Article

C2 - 9774548

AN - SCOPUS:0032170325

SN - 1047-8477

VL - 123

SP - 83

EP - 85

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 1

ER -

Preliminary crystallographic study of glutathione S-transferase fused with the nuclear matrix targeting signal of the transcription factor AML- 1/CBF-α2

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this