Preeclampsia/Eclampsia candidate genes show altered methylation in maternal leukocytes of preeclamptic women at the time of delivery

Wendy M. White; Zhifu Sun; Kristi S. Borowski; Brian C. Brost; Norman P. Davies; Carl H. Rose; Vesna D. Garovic

doi:10.3109/10641955.2016.1162315

Preeclampsia/Eclampsia candidate genes show altered methylation in maternal leukocytes of preeclamptic women at the time of delivery

Wendy M. White, Zhifu Sun, Kristi S. Borowski, Brian C. Brost, Norman P. Davies, Carl H. Rose, Vesna D. Garovic

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Objective: To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery. Methods: A matched case–control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL (n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32). Results: PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL. Conclusion: Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.

Original language	English (US)
Pages (from-to)	394-404
Number of pages	11
Journal	Hypertension in Pregnancy
Volume	35
Issue number	3
DOIs	https://doi.org/10.3109/10641955.2016.1162315
State	Published - Jul 2 2016

Keywords

AGT
CALCA
DDAH1
DNA methylation
POMC
candidate gene
leukocyte
preeclampsia
pregnancy

ASJC Scopus subject areas

Internal Medicine
Obstetrics and Gynecology

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10.3109/10641955.2016.1162315

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title = "Preeclampsia/Eclampsia candidate genes show altered methylation in maternal leukocytes of preeclamptic women at the time of delivery",

abstract = "Objective: To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery. Methods: A matched case–control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL (n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32). Results: PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL. Conclusion: Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.",

keywords = "AGT, CALCA, DDAH1, DNA methylation, POMC, candidate gene, leukocyte, preeclampsia, pregnancy",

author = "White, {Wendy M.} and Zhifu Sun and Borowski, {Kristi S.} and Brost, {Brian C.} and Davies, {Norman P.} and Rose, {Carl H.} and Garovic, {Vesna D.}",

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doi = "10.3109/10641955.2016.1162315",

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T1 - Preeclampsia/Eclampsia candidate genes show altered methylation in maternal leukocytes of preeclamptic women at the time of delivery

AU - White, Wendy M.

AU - Sun, Zhifu

AU - Borowski, Kristi S.

AU - Brost, Brian C.

AU - Davies, Norman P.

AU - Rose, Carl H.

AU - Garovic, Vesna D.

PY - 2016/7/2

Y1 - 2016/7/2

N2 - Objective: To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery. Methods: A matched case–control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL (n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32). Results: PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL. Conclusion: Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.

AB - Objective: To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery. Methods: A matched case–control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL (n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32). Results: PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL. Conclusion: Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.

KW - AGT

KW - CALCA

KW - DDAH1

KW - DNA methylation

KW - POMC

KW - candidate gene

KW - leukocyte

KW - preeclampsia

KW - pregnancy

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Preeclampsia/Eclampsia candidate genes show altered methylation in maternal leukocytes of preeclamptic women at the time of delivery

Abstract

Keywords

ASJC Scopus subject areas

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