Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response

Diana Jurk, Chunfang Wang, Satomi Miwa, Mandy Maddick, Viktor Korolchuk, Avgi Tsolou, Efstathios S. Gonos, Christopher Thrasivoulou, M. Jill Saffrey, Kerry Cameron, Thomas von Zglinicki

Research output: Contribution to journalArticlepeer-review

244 Scopus citations


In senescent cells, a DNA damage response drives not only irreversible loss of replicative capacity but also production and secretion of reactive oxygen species (ROS) and bioactive peptides including pro-inflammatory cytokines. This makes senescent cells a potential cause of tissue functional decline in aging. To our knowledge, we show here for the first time evidence suggesting that DNA damage induces a senescence-like state in mature postmitotic neurons in vivo. About 40-80% of Purkinje neurons and 20-40% of cortical, hippocampal and peripheral neurons in the myenteric plexus from old C57Bl/6 mice showed severe DNA damage, activated p38MAPkinase, high ROS production and oxidative damage, interleukin IL-6 production, heterochromatinization and senescence-associated β-galactosidase activity. Frequencies of these senescence-like neurons increased with age. Short-term caloric restriction tended to decrease frequencies of positive cells. The phenotype was aggravated in brains of late-generation TERC-/- mice with dysfunctional telomeres. It was fully rescued by loss of p21(CDKN1A) function in late-generation TERC-/-CDKN1A-/- mice, indicating p21 as the necessary signal transducer between DNA damage response and senescence-like phenotype in neurons, as in senescing fibroblasts and other proliferation-competent cells. We conclude that a senescence-like phenotype is possibly not restricted to proliferation-competent cells. Rather, dysfunctional telomeres and/or accumulated DNA damage can induce a DNA damage response leading to a phenotype in postmitotic neurons that resembles cell senescence in multiple features. Senescence-like neurons might be a source of oxidative and inflammatory stress and a contributor to brain aging.

Original languageEnglish (US)
Pages (from-to)996-1004
Number of pages9
JournalAging Cell
Issue number6
StatePublished - Dec 2012


  • Aging
  • Brain
  • Inflammation
  • Neurons
  • Oxidative stress
  • Senescence

ASJC Scopus subject areas

  • Aging
  • Cell Biology


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